Wei Zheng-xin, Guo Dong-qin, Li Hai-feng, Ding Bo, Zhang Jie, Zhou Nong, Yu Jie
Zhongguo Zhong Yao Za Zhi. 2015 Oct;40(20):3945-52.
Through potted inoculation test at room temperature and indoor analysis, the photosynthetic parameters and physiological and biochemical indexes of Paris polyphylla var. yunnanensis were observed after 28 arbuscular mycorrhizal (AM) fungi were injected into the P. polyphylla var. yunnanensis growing in a sterile soil environment. The results showed that AM fungi established a good symbiosis with P. polyphylla var. yunnanensis. The AM fungi influenced the photosynthetic parameters and physiological and biochemical indexes of P. polyphylla var. yunnanensis. And the influences were varied depending on different AM fungi. The application of AM fungi improved photosynthesis intensity of P. polyphylla var. yunnanensis mesophyll cells, the contents of soluble protein and soluble sugar, protective enzyme activity of P. polyphylla var. yunnanensis leaf, which was beneficial to resist the adverse environment and promote the growth of P. polyphylla var. yunnanensis. Otherwise, there was a certain mutual selectivity between P. polyphylla var. yunnanensis and AM fungi. From the comprehensive effect of inoculation, Racocetra coralloidea, Scutellospora calospora, Claroideoglomus claroideum, S. pellucida and Rhizophagus clarus were the most suitable AM fungi to P. polyphylla var. yunnanensis when P. polyphylla var. yunnanensis was planted in the field.
通过室温盆栽接种试验及室内分析,在无菌土壤环境中生长的滇重楼植株内接种28种丛枝菌根(AM)真菌后,观测滇重楼的光合参数及生理生化指标。结果表明,AM真菌与滇重楼建立了良好的共生关系。AM真菌对滇重楼的光合参数及生理生化指标产生了影响,且不同AM真菌的影响存在差异。AM真菌的施用提高了滇重楼叶肉细胞的光合强度、可溶性蛋白和可溶性糖含量以及滇重楼叶片的保护酶活性,有利于抵御逆境并促进滇重楼生长。此外,滇重楼与AM真菌之间存在一定的相互选择性。从接种的综合效果来看,当滇重楼进行田间种植时,珊瑚状无梗囊霉、美丽盾巨孢囊霉、明球囊霉、透明盾巨孢囊霉和明线球囊霉是最适合滇重楼的AM真菌。