Wang Li-Feng, Chen Hai-Jin, Yu Jin-Long, Qi Jia, Lin Xiao-Hua, Zou Zhao-Wei
Department of General Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou 510280, China.E-mail:
Nan Fang Yi Ke Da Xue Xue Bao. 2016 Mar;36(3):396-400.
To explore the expression of Rictor and mTOR in the colorectal cancer and their clinical significance.
The expression levels of Rictor and mTOR in HCT116, SW480, LoVo and HCoEpiC cells were detected by indirect immunofluorescence and Western blotting. Sixty-two paraffin-embedded surgical specimens of colorectal cancer tissue and adjacent tissues were examined for Rictor expression using immunohistochemistry. The association of the expression levels of Rictor protein with the clinicopathologic features and the overall survival of the patients was analyzed.
The expression level of Rictor was significantly higher in colorectal cancer tissues than in the adjacent tissues (P<0.05). The expression levels of Rictor and mTOR in the colon cancer cell lines were higher than those in human normal colon epithelial cell line HCoEpiC. The expression of Rictor was correlated with Dukes stage and lymphatic metastasis of the tumors but not with other clinicopathological parameter (P>0.05). Patients with Rictor expression had a lower overall survival rate than those without Rictor expression.
Rictor overexpression is associated with the carcinogenesis and progression of colorectal cancer and can be an independent indicator for evaluating the prognosis of colorectal cancer patients.
探讨Rictor和mTOR在结直肠癌中的表达及其临床意义。
采用间接免疫荧光法和蛋白质免疫印迹法检测HCT116、SW480、LoVo细胞及人正常结肠上皮细胞系HCoEpiC中Rictor和mTOR的表达水平。采用免疫组织化学法检测62例结直肠癌组织及癌旁组织石蜡包埋标本中Rictor的表达情况。分析Rictor蛋白表达水平与患者临床病理特征及总生存的相关性。
结直肠癌组织中Rictor的表达水平显著高于癌旁组织（P<0.05）。结肠癌细胞系中Rictor和mTOR的表达水平高于人正常结肠上皮细胞系HCoEpiC。Rictor的表达与肿瘤的Dukes分期和淋巴结转移相关，而与其他临床病理参数无关（P>0.05）。有Rictor表达的患者总生存率低于无Rictor表达的患者。
Rictor过表达与结直肠癌的发生发展相关，可作为评估结直肠癌患者预后的独立指标。
