Uçar Fahri, Sönmez Mehmet, Ermantaş Nilay, Özbaş Hasan Mücahit, Cansız Abide, Balcı Mustafa, Yılmazz Mustafa
Akdeniz University, School of Medicine, Department of Medical Biology & Genetics, Antalya, Turkey.
Karadeniz Technical University, School of Medicine, Department of Internal Medicine Division of Haematology, Trabzon, Turkey.
Gene. 2016 Jul 25;586(2):263-7. doi: 10.1016/j.gene.2016.04.017. Epub 2016 Apr 7.
A significant association between lymphomas and HLA alleles has been shown in previous studies. However, the frequency of HLA alleles and haplotypes has not been studied in Turkish lymphoma patients. We studied HLA-A, -B, -DRB1 alleles and haplotypes in 80 adult lymphomas and 360 unrelated normal subjects by PCR-SSOP method using Luminex technology. The allele frequencies of HLA-A29, B07, and DRB111 were higher in patients with Hodgkin's lymphoma (HL) compared with the controls [OR; 5.65 (95%CI; 2.16-14.81), P=0.001], [OR; 3.00 (95%CI; 1.50-5.99), P=0.003)], and [OR; 1.80 (95%CI; 1.08-3.01), P=0.002); respectively]. In patients with non-Hodgkin's lymphoma (NHL) HLA-B51 and DRB104 allele frequencies were higher than controls [OR; 2.25 (95%CI; 1.27-4.00), P=0.007] and [OR; 2.14 (95%CI; 1.20-3.78), P=0.01]. The most frequently observed haplotypes were A02 B35 DRB111 (7.50% vs. 1.89%) in HL patients, A02 B51 DRB111 (5.00% vs. 1.96%) in NHL patients, and A02 B35 DRB113 (2.19%) in the controls. We detected four haplotypes specific to NHL, five haplotypes to HL patients. Seven haplotypes were unique to controls. Our findings suggest that in HL patients, HLA-A29, B07, and DRB111 alleles, and in NHL patients, HLA-B51 and DRB1*04 alleles might be presumptive predisposing factors.
先前的研究已表明淋巴瘤与人类白细胞抗原（HLA）等位基因之间存在显著关联。然而，土耳其淋巴瘤患者中HLA等位基因和单倍型的频率尚未得到研究。我们采用基于Luminex技术的聚合酶链反应-序列特异性寡核苷酸探针（PCR-SSOP）方法，对80例成年淋巴瘤患者和360名无关正常受试者的HLA-A、-B、-DRB1等位基因及单倍型进行了研究。与对照组相比，霍奇金淋巴瘤（HL）患者中HLA-A29、B07和DRB111的等位基因频率更高[比值比（OR）；5.65（95%可信区间（CI）；2.16 - 14.81），P = 0.001]，[OR；3.00（95%CI；1.50 - 5.99），P = 0.003]，以及[OR；1.80（95%CI；1.08 - 3.01），P = 0.002]；非霍奇金淋巴瘤（NHL）患者中HLA-B51和DRB104等位基因频率高于对照组[OR；2.25（95%CI；1.27 - 4.00），P = 0.007]和[OR；2.14（95%CI；1.20 - 3.78），P = 0.01]。HL患者中最常观察到的单倍型是A02 B35 DRB111（7.50%对1.89%），NHL患者中是A02 B51 DRB111（5.00%对1.96%），对照组中是A02 B35 DRB113（2.19%）。我们检测到四种NHL特异性单倍型，五种HL患者特异性单倍型。七种单倍型是对照组所特有的。我们的研究结果表明，在HL患者中，HLA-A29、B07和DRB111等位基因，以及在NHL患者中，HLA-B51和DRB1*04等位基因可能是推定的易感因素。
