Pucci P, Ferranti P, Marino G, Malorni A
Dipartimento di Chimica Organica e Biologica dell'Università, Naples, Italy.
Biomed Environ Mass Spectrom. 1989 Jan;18(1):20-6. doi: 10.1002/bms.1200180105.
A procedure using fast atom bombardment mass spectrometry for mapping the proteolytic digest of alpha-, beta- and gamma-globin chains of normal human haemoglobin was developed. It required the separation of globins prior to their analysis by mass spectrometry of their enzymatic digests. Almost all the expected peptides were identified by direct analysis of the peptide mixture. Peptide recognition along the globin chain sequences was easily made on the basis of their molecular weight and the assignments performed were confirmed submitting the whole peptide mixture to a single step of Edman degradation. The procedure was successfully applied to the structural characterization of three variant human beta-globin chains, demonstrating the general applicability of this mapping procedure in the analysis of haemoglobinopathies.
开发了一种使用快原子轰击质谱法绘制正常人血红蛋白α、β和γ珠蛋白链蛋白水解消化图谱的方法。该方法要求在通过酶解产物的质谱分析之前对珠蛋白进行分离。通过对肽混合物的直接分析，几乎鉴定出了所有预期的肽段。根据肽段的分子量，很容易在珠蛋白链序列上识别肽段，并且通过将整个肽混合物进行一步埃德曼降解来确认所进行的归属。该方法成功应用于三种变异人β珠蛋白链的结构表征，证明了这种图谱绘制方法在血红蛋白病分析中的普遍适用性。
