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一种用于布氏锥虫的香草酸诱导表达系统。

A vanillic acid inducible expression system for Trypanosoma brucei.

作者信息

Sunter Jack D

机构信息

Sir William Dunn School of Pathology, University of Oxford, South Parks Road, OX1 3RE, UK.

出版信息

Mol Biochem Parasitol. 2016 May;207(1):45-8. doi: 10.1016/j.molbiopara.2016.04.001. Epub 2016 Apr 7.

DOI:10.1016/j.molbiopara.2016.04.001
PMID:27063979
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4920640/
Abstract

Reverse genetics in Trypanosoma brucei is dependent on the tetracycline inducible system for the precise control over the expression of both genes and dsRNA. Another independent inducible system for trypanosomes would enable the control of the activities of two different genes in the same cell, providing greater experimental sophistication. Here, I describe the development of the vanillic acid based inducible expression system for T. brucei, which operates independently of, and can be used in parallel with the tetracycline inducible system.

摘要

布氏锥虫的反向遗传学依赖于四环素诱导系统来精确控制基因和双链RNA的表达。另一种针对锥虫的独立诱导系统将能够控制同一细胞中两个不同基因的活性，从而提供更高的实验复杂性。在此，我描述了基于香草酸的布氏锥虫诱导表达系统的开发，该系统独立于四环素诱导系统运行，并且可以与之并行使用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/714f/4920640/76fbf7c3037e/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/714f/4920640/55d25df2f6ca/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/714f/4920640/c625e8fc5ac4/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/714f/4920640/76fbf7c3037e/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/714f/4920640/55d25df2f6ca/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/714f/4920640/c625e8fc5ac4/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/714f/4920640/76fbf7c3037e/gr2.jpg

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本文引用的文献

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Antigenic variation in African trypanosomes.非洲锥虫的抗原变异
Mol Biochem Parasitol. 2014 Jul;195(2):123-9. doi: 10.1016/j.molbiopara.2014.05.001. Epub 2014 May 22.
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A modular and optimized single marker system for generating Trypanosoma brucei cell lines expressing T7 RNA polymerase and the tetracycline repressor.一种用于生成表达 T7 RNA 聚合酶和四环素阻遏物的布鲁氏锥虫细胞系的模块化和优化的单一标记系统。
在体外和体内可靠、可扩展的血液期刚果锥虫功能遗传学研究。
PLoS Pathog. 2021 Jan 22;17(1):e1009224. doi: 10.1371/journal.ppat.1009224. eCollection 2021 Jan.
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Non-linear hierarchy of the quorum sensing signalling pathway in bloodstream form African trypanosomes.血液循环期非洲锥虫群体感应信号通路的非线性层级结构。
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A Riboswitch-based Inducible Gene Expression System for Trypanosoma brucei.一种基于核糖开关的布氏锥虫诱导型基因表达系统。
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