丁基羟基甲苯添加对哈里亚纳公牛冻融后精子活力、运动性和膜完整性的影响。
Effect of supplementation of butylated hydroxytoluene on post-thaw sperm viability, motility and membrane integrity of Hariana bulls.
作者信息
Patel Akhil, Saxena Atul, Swain Dilip Kumar, Yadav Dushyant, Yadav Sanjay Singh, Kumar Abhishek, Kumar Anuj
机构信息
Department of Veterinary Obstetrics & Gynaecology, College of Veterinary Science & Animal Husbandry, U.P. Pandit Deendayal Upadhayaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan, Mathura - 281 001, Uttar Pradesh, India.
Department of Veterinary Physiology, College of Veterinary Science & Animal Husbandry, U.P. Pandit Deendayal Upadhayaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan, Mathura - 281 001, Uttar Pradesh, India.
出版信息
Vet World. 2015 Jun;8(6):808-12. doi: 10.14202/vetworld.2015.808-812. Epub 2015 Jun 30.
AIM
This study was aimed to see the beneficial effect of butylated hydroxytoluene (BHT) as a semen additive of Hariana bull semen.
MATERIALS AND METHODS
The study was carried out in Hariana bulls. Twenty-four ejaculates from two bulls were used for this study. Each ejaculate was extended with standard glycerolated egg yolk tris extender and supplemented with BHT at two concentrations as 0.5 mM (T1) and 1.0 mM (T2). After dilution, equilibration and 24 h of cryopreservation, the samples were analyzed for progressive motility, sperm viability and membrane integrity.
RESULTS
Progressive motility, sperm viability and sperm membrane integrity were significantly (p<0.05) increased in the samples fortified with BHT as compared to the control during the process of cryopreservation and thawing. The BHT concentration of 1 mM revealed better results as compared to 0.5 mM.
CONCLUSION
Addition of 1.0 mM BHT was found better in cryopreservation of Hariana bull semen compared to 0.5 mM BHT and control samples. The addition of BHT has improved the sperm quality by acting as an antioxidant thereby reducing the lipid peroxidation of the sperms.
目的
本研究旨在观察丁基羟基甲苯（BHT）作为哈里亚纳公牛精液添加剂的有益效果。
材料与方法
本研究在哈里亚纳公牛身上进行。使用来自两头公牛的24份精液样本。每份精液样本均用标准甘油化蛋黄三羟甲基氨基甲烷稀释液进行稀释，并添加两种浓度的BHT，分别为0.5 mM（T1）和1.0 mM（T2）。稀释、平衡及冷冻保存24小时后，对样本进行精子前向运动率、精子活力及膜完整性分析。
结果
在冷冻保存和解冻过程中，与对照组相比，添加BHT的样本中精子前向运动率、精子活力及精子膜完整性均显著提高（p<0.05）。1 mM BHT浓度组的效果优于0.5 mM BHT浓度组。
结论
与0.5 mM BHT及对照组样本相比，添加1.0 mM BHT对哈里亚纳公牛精液的冷冻保存效果更佳。BHT作为抗氧化剂，通过减少精子的脂质过氧化反应，提高了精子质量。
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