Söderbäck M, Hansson E, Tottmar O, Rönnbäck L
Cell Mol Biol. 1989;35(1):1-16.
Primary cultures from 15-17 days old fetal rat cerebral cortex, striatum, hippocampus, substantia nigra and brain stem were grown for ten days. Cell aggregates were formed one to two days after seeding. The cell bodies migrated peripherally from the clusters during development and networks of processes were formed. The cultures from the different brain regions contained predominantly neurons, stained by an antiserum against the neuron-specific enolase (NSE). There were differences in morphological appearance of the aggregates and also of the single neuronal cells cultivated from the various brain regions. On the bottom of the culture dishes a monolayer was formed of predominantly undifferentiated (mesenchymal-like) cells. Some cells of the monolayer stained for the astrocyte markers glial fibrillary acidic protein (GFAp) or S-100. The majority of the cells were, however, unstained to these markers. Very few endothelial cells and macrophages were observed.
来自15 - 17日龄胎鼠大脑皮层、纹状体、海马体、黑质和脑干的原代培养物培养了10天。接种后1至2天形成细胞聚集体。在发育过程中，细胞体从细胞簇向周边迁移，并形成了神经突网络。来自不同脑区的培养物主要包含神经元，用抗神经元特异性烯醇化酶（NSE）的抗血清染色。从不同脑区培养的聚集体以及单个神经元细胞的形态外观存在差异。在培养皿底部形成了一层主要由未分化（间充质样）细胞组成的单层。单层中的一些细胞用星形胶质细胞标志物胶质纤维酸性蛋白（GFAp）或S - 100染色。然而，大多数细胞对这些标志物未染色。观察到极少数内皮细胞和巨噬细胞。
