牛垂体中间部体外合成β-内啡肽、γ-脂蛋白和β-促脂解素。
In vitro biosynthesis of beta-endorphin, gamma-lipoprotein, and beta-lipotropin by the pars intermedia of beef pituitary glands.
作者信息
Crine P, Benjannet S, Seidah N G, Lis M, Chrétien M
出版信息
Proc Natl Acad Sci U S A. 1977 Oct;74(10):4276-80. doi: 10.1073/pnas.74.10.4276.
Abstract
Labeled amino acids were incorporated with proteins during incubations of isolated cells from the pars intermedia of beef pituitary glands. After 3 hr of incubation with methionine and [3H]lysine, approximately equivalent amounts of labeled gamma-lipotropin and beta-endorphins were isolated. They were found to be major synthesis products of the pars intermedia. In contrast, very little labeled beta-lipotropin was recovered. Another major synthesis product of the pars intermedia was also purified. Its partial amino acid sequence and molecular weight were determined and it was concluded that this peptide cannot be identified as any known pituitary hormone or protein fragment.
摘要
在对牛垂体中间部的分离细胞进行孵育期间，标记的氨基酸与蛋白质结合。在用甲硫氨酸和[³H]赖氨酸孵育3小时后，分离出了大致等量的标记γ-促脂素和β-内啡肽。它们被发现是中间部的主要合成产物。相比之下，回收的标记β-促脂素很少。中间部的另一种主要合成产物也被纯化。确定了其部分氨基酸序列和分子量，并得出结论，该肽不能被鉴定为任何已知的垂体激素或蛋白质片段。
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