Pati A R, Patton W N, Harris R I
Department of Haematology, Coventry and Warwickshire Hospital, UK.
Clin Lab Haematol. 1989;11(1):27-30. doi: 10.1111/j.1365-2257.1989.tb00171.x.
Blood samples from 21 patients with confirmed hereditary spherocytosis (HS) were studied using the Technicon H1 blood counter. Nine patients had had a splenectomy and 12 were unsplenectomized. In the latter, the means of MCHC and RDW as measured by the H1 were significantly higher than results obtained using the Technicon H6000. Furthermore, spherocytes were recognizable in all of the H1 histograms of red cell size and Hb concentration as distinctive hyperchromic and microcytic tails, and the HDW (Hb distribution width) was consistently elevated. In the splenectomized patients, although the mean MCHC and RDW results were significantly higher as measured by the H1, it was not possible to recognize any abnormalities in the red cell histograms, and the HDW results were normal. Two of 75 apparently normal blood donors showed very small tails of hyperchromic cells, but in neither was this sufficient to disturb the MCHC, RDW or HDW parameters, and confusion with an unsplenectomized case of HS would be very unlikely. The H1 appearances of HS are so specific that the measurement of osmotic fragility will seldom be necessary.
使用Technicon H1血液计数器对21例确诊为遗传性球形红细胞增多症（HS）患者的血样进行了研究。9例患者已行脾切除术，12例未行脾切除术。在未行脾切除术的患者中，H1测得的平均MCHC和RDW显著高于使用Technicon H6000获得的结果。此外，在所有H1红细胞大小和血红蛋白浓度直方图中，球形红细胞均表现为明显的高色素性和小细胞性尾巴，且HDW（血红蛋白分布宽度）持续升高。在脾切除患者中，尽管H1测得的平均MCHC和RDW结果显著更高，但在红细胞直方图中无法识别任何异常，且HDW结果正常。75名看似正常的献血者中有2名显示出非常小的高色素细胞尾巴，但这两者均不足以干扰MCHC、RDW或HDW参数，且极不可能与未行脾切除术的HS病例混淆。HS的H1表现非常特异，因此很少需要测量渗透脆性。
