波形蛋白调节非小细胞肺癌的侵袭性且是不良预后标志物。
Vimentin Regulates Invasiveness and Is a Poor Prognostic Marker in Non-small Cell Lung Cancer.
作者信息
Tadokoro Akira, Kanaji Nobuhiro, Liu Dage, Yokomise Hiroyasu, Haba Reiji, Ishii Tomoya, Takagi Takehiro, Watanabe Naoki, Kita Nobuyuki, Kadowaki Norimitsu, Bandoh Shuji
机构信息
Department of Internal Medicine, Division of Hematology, Rheumatology and Respiratory Medicine, Faculty of Medicine, Kagawa University, Kagawa, Japan
Department of Internal Medicine, Division of Hematology, Rheumatology and Respiratory Medicine, Faculty of Medicine, Kagawa University, Kagawa, Japan.
出版信息
Anticancer Res. 2016 Apr;36(4):1545-51.
BACKGROUND
Lung cancer cells often express vimentin. However, the function of vimentin in lung cancer cells has not been fully evaluated.
MATERIALS AND METHODS
We evaluated the association between vimentin expression in resected non-small cell lung cancer (NSCLC) specimens and prognosis. Short-interfering RNA targeting vimentin and establishment of an invasive cell line by repeated selection of invasive cells using a Matrigel membrane invasion chamber system (MICS) were performed. MICS was used to reveal the relationship between invasiveness and vimentin.
RESULTS
Vimentin positivity was significantly associated with a poor prognosis and was significantly lower in squamous cell carcinoma than in adenocarcinoma. In in vitro experiments, silencing of vimentin reduced invasiveness. Highly invasive cell lines exhibited higher expression of vimentin than did parental cells, and invasive ability was reduced by knockdown of vimentin.
CONCLUSION
Vimentin expression is associated with prognosis via alteration of the invasive ability of NSCLC cells.
背景
肺癌细胞常表达波形蛋白。然而，波形蛋白在肺癌细胞中的功能尚未得到充分评估。
材料与方法
我们评估了切除的非小细胞肺癌（NSCLC）标本中波形蛋白表达与预后之间的关联。使用针对波形蛋白的短干扰RNA，并通过使用基质胶膜侵袭室系统（MICS）反复选择侵袭性细胞来建立侵袭性细胞系。利用MICS揭示侵袭性与波形蛋白之间的关系。
结果
波形蛋白阳性与预后不良显著相关，且在鳞状细胞癌中的表达明显低于腺癌。在体外实验中，波形蛋白的沉默降低了侵袭性。高侵袭性细胞系比亲代细胞表现出更高的波形蛋白表达，并且波形蛋白的敲低降低了侵袭能力。
结论
波形蛋白表达通过改变NSCLC细胞的侵袭能力与预后相关。
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