Olsen Ingrid H, Langer Seppo W, Federspiel Birgitte H, Oxbøl Jytte, Loft Annika, Berthelsen Anne Kiil, Mortensen Jann, Oturai Peter, Knigge Ulrich, Kjær Andreas
European NET Centre of Excellence, Rigshospitalet, University of CopenhagenDenmark; Department of Clinical Physiology, Nuclear Medicine & PET and Cluster for Molecular Imaging, Rigshospitalet and University of CopenhagenDenmark; Department of Endocrinology, Rigshospitalet, University of CopenhagenDenmark; Department of Surgical Gastroenterology, Rigshospitalet, University of CopenhagenDenmark; Department of Oncology, Rigshospitalet, University of CopenhagenDenmark; Department of Pathology, Rigshospitalet, University of CopenhagenDenmark.
European NET Centre of Excellence, Rigshospitalet, University of CopenhagenDenmark; Department of Oncology, Rigshospitalet, University of CopenhagenDenmark.
Am J Nucl Med Mol Imaging. 2016 Jan 28;6(1):59-72. eCollection 2016.
Somatostatin receptor expression on both protein and gene expression level was compared with in vivo (68)Ga-DOTATOC PET/CT in patients with neuroendocrine carcinomas (NEC). Twenty-one patients with verified NEC who underwent a (68)Ga-DOTATOC PET/CT between November 2012 and May 2014, were retrospectively included. By real-time polymerase chain reaction, we quantitatively determined the gene expression of several genes and compared with (68)Ga-DOTATOC PET uptake. By immunohistochemistry we qualitatively studied the expression of assorted proteins in NEC. The median age at diagnosis was 68 years (range 41-84) years. All patients had WHO performance status 0-1. Median Ki67 index was 50% (range 20-100%). Gene expression of somatostatin receptor subtype (SSTR) 2 and Ki67 were both positively correlated to the (68)Ga-DOTATOC uptake (r=0.89; p<0.0001 and r=0.5; p=0.021, respectively). Furthermore, SSTR2 and SSTR5 gene expression were strongly and positively correlated (r=0.57; p=0.006). This study as the first verifies a positive and close correlation of (68)Ga-DOTATOC uptake and gene expression of SSTR2 in NEC. SSTR2 gene expression has a stronger correlation to (68)Ga-DOTATOC uptake than SSTR5. In addition, the results indicate that the gene expression levels of SSTR2 and SSTR5 at large follow one another.
在神经内分泌癌（NEC）患者中，将生长抑素受体在蛋白质和基因表达水平上的表达与体内（68）Ga-DOTATOC PET/CT进行了比较。回顾性纳入了2012年11月至2014年5月期间接受（68）Ga-DOTATOC PET/CT检查且确诊为NEC的21例患者。通过实时聚合酶链反应，我们定量测定了几种基因的基因表达，并与（68）Ga-DOTATOC的摄取情况进行了比较。通过免疫组织化学，我们定性研究了NEC中各种蛋白质的表达。诊断时的中位年龄为68岁（范围41-84岁）。所有患者的世界卫生组织体能状态均为0-1级。Ki67指数中位数为50%（范围20-100%）。生长抑素受体亚型（SSTR）2的基因表达和Ki67均与（68）Ga-DOTATOC的摄取呈正相关（r分别为0.89；p<0.0001和r为0.5；p=0.021）。此外，SSTR2和SSTR5基因表达呈强正相关（r=0.57；p=0.006）。本研究首次证实了NEC中（68）Ga-DOTATOC摄取与SSTR2基因表达呈正相关且密切相关。SSTR2基因表达与（68）Ga-DOTATOC摄取的相关性比SSTR5更强。此外，结果表明SSTR2和SSTR5的基因表达水平在很大程度上相互跟随。
