Wu Guocai, Liu Xia, Liu Song, Shu Jun, Sun Zhifu, Luo Bing
Department of Medical Microbiology, Qingdao University Medical College, Qingdao, PR China.
Intervirology. 2015;58(6):386-92. doi: 10.1159/000444920. Epub 2016 Apr 13.
The aim of this study was to characterize the specific Epstein-Barr virus (EBV) polymorphisms from nasopharyngeal carcinoma (NPC) patients and healthy donors in Southern China, and to explore the relationship between EBV genotypes and NPC.
The EBNA-2 gene of 32 EBV-positive NPCs and 39 EBV-positive throat washing (TW) samples from healthy individuals from Southern China was analyzed using PCR and sequencing.
We found E2-A to be the predominant subtype in Southern China. The distribution of EBNA-2 subtypes between NPCs and TWs was significantly different (p < 0.01) and the E2-A subtype was overly represented in NPCs.
E2-A is the predominant subtype in Southern China, and the distribution of EBNA-2 subtypes between NPCs and TWs is significantly different. In Southern China, the E2-A subtype is significantly more frequent in NPCs than TWs, and is also more common than in Northern China. This suggests that the E2-A subtype may play an important role in the pathogenesis of NPC and that EBNA-2 gene variations are tumor-specific polymorphisms.
本研究旨在鉴定中国南方鼻咽癌（NPC）患者和健康供者中特定的爱泼斯坦-巴尔病毒（EBV）多态性，并探讨EBV基因型与NPC之间的关系。
采用聚合酶链反应（PCR）和测序技术，分析来自中国南方的32例EBV阳性NPC患者和39例健康个体的EBV阳性咽洗液（TW）样本中的EBNA-2基因。
我们发现E2-A是中国南方的主要亚型。NPC患者和TW样本中EBNA-2亚型的分布存在显著差异（p < 0.01），且E2-A亚型在NPC患者中过度表达。
E2-A是中国南方的主要亚型，NPC患者和TW样本中EBNA-2亚型的分布存在显著差异。在中国南方，NPC患者中E2-A亚型的频率显著高于TW样本，且也比中国北方更常见。这表明E2-A亚型可能在NPC的发病机制中起重要作用，且EBNA-2基因变异是肿瘤特异性多态性。
