2-氨基嘌呤诱导T4噬菌体的诱变作用：一个将突变频率与DNA中2-氨基嘌呤掺入量相关联的模型。
2-Aminopurine-induced mutagenesis in T4 bacteriophage: a model relating mutation frequency to 2-aminopurine incorporation in DNA.
作者信息
Goodman M F, Hopkins R, Gore W C
出版信息
Proc Natl Acad Sci U S A. 1977 Nov;74(11):4806-10. doi: 10.1073/pnas.74.11.4806.
Abstract
We measured the in vivo incorporation of 2-aminopurine into DNA of T4 bacteriophage allelic for gene 43 (DNA polymerase), mutator (L56), 43+, and antimutator (L141). The magnitude of incorporation (mol/mol of Thy) was 1/1500 in L56, 1/1600 in 43+, and 1/8900 in L141. The incorporation ratio L56:43+:L141 in vivo was equal to that mediated by the purified DNA polymerases of these allelic phages in vitro. A model for 2-aminopurine-induced A-T in equilibrium G-C transitions is discussed. The model is used to predict the magnitudes of replication errors (C mispairing with a template 2-aminopurine) and incorporation errors (2-aminopurine mispairing with a template C) per round of replication and to investigate the asymmetry in 2-aminopurine-induced transitions favoring the A-T leads to G-C pathway over G-C leads to A-T. We suggest that the fidelity of L56 and L141 DNA polymerases exemplifies one-step and two-step editing, respectively.
摘要
我们测定了2-氨基嘌呤在T4噬菌体基因43（DNA聚合酶）、诱变型（L56）、野生型（43+）和抗诱变型（L141）等位基因的DNA中的体内掺入情况。掺入量（每摩尔胸腺嘧啶的摩尔数）在L56中为1/1500，在43+中为1/1600，在L141中为1/8900。2-氨基嘌呤在体内的掺入比例L56:43+:L141与这些等位基因噬菌体的纯化DNA聚合酶在体外介导的比例相同。讨论了2-氨基嘌呤诱导的A-T与G-C平衡转换的模型。该模型用于预测每轮复制中复制错误（胞嘧啶与模板2-氨基嘌呤错配）和掺入错误（2-氨基嘌呤与模板胞嘧啶错配）的大小，并研究2-氨基嘌呤诱导的转换中有利于A-T到G-C途径而非G-C到A-T途径的不对称性。我们认为，L56和L141 DNA聚合酶的保真度分别体现了一步编辑和两步编辑。
相似文献
1
2-Aminopurine-induced mutagenesis in T4 bacteriophage: a model relating mutation frequency to 2-aminopurine incorporation in DNA.2-氨基嘌呤诱导T4噬菌体的诱变作用：一个将突变频率与DNA中2-氨基嘌呤掺入量相关联的模型。
Proc Natl Acad Sci U S A. 1977 Nov;74(11):4806-10. doi: 10.1073/pnas.74.11.4806.
2
Error induction and correction by mutant and wild type T4 DNA polymerases. Kinetic error discrimination mechanisms.突变型和野生型T4 DNA聚合酶的错误诱导与校正。动力学错误识别机制。
J Biol Chem. 1979 Mar 25;254(6):1902-12.
3
Nonrandom substitution of 2-aminopurine for adenine during deoxyribonucleic acid synthesis in vitro.体外脱氧核糖核酸合成过程中2-氨基嘌呤对腺嘌呤的非随机取代
Biochemistry. 1981 Oct 13;20(21):6235-44. doi: 10.1021/bi00524a051.
4
5
The biochemical basis of 5-bromouracil- and 2-aminopurine-induced mutagenesis.
Basic Life Sci. 1985;31:409-23. doi: 10.1007/978-1-4613-2449-2_25.
6
Influence of local nucleotide sequence on substitution of 2-aminopurine for adenine during deoxyribonucleic acid synthesis in vitro.体外脱氧核糖核酸合成过程中局部核苷酸序列对2-氨基嘌呤取代腺嘌呤的影响。
Biochemistry. 1983 Oct 11;22(21):4905-15. doi: 10.1021/bi00290a006.
7
On the fidelity of DNA replication. The accuracy of T4 DNA polymerases in copying phi X174 DNA in vitro.关于DNA复制的保真度。T4 DNA聚合酶在体外复制φX174 DNA的准确性。
J Biol Chem. 1984 Feb 10;259(3):1539-45.
8
Influence of diverse gene 43 DNA polymerases on the incorporation and replication in vivo of 2-aminopurine at A X T base-pairs in bacteriophage T4.多种基因43 DNA聚合酶对噬菌体T4中腺嘌呤与胸腺嘧啶碱基对处2-氨基嘌呤在体内掺入和复制的影响。
J Mol Biol. 1981 Aug 5;150(2):197-216. doi: 10.1016/0022-2836(81)90449-6.
9
10
Site specificity and variability in the mutator and antimutator effects of phage T4 gene 43 mutants.噬菌体T4基因43突变体的诱变和抗诱变效应中的位点特异性和变异性。
Genetics. 1978 Dec;90(4):647-57. doi: 10.1093/genetics/90.4.647.
引用本文的文献
1
Mismatch repair causes the dynamic release of an essential DNA polymerase from the replication fork.错配修复导致一种必需的 DNA 聚合酶从复制叉上动态释放。
Mol Microbiol. 2011 Nov;82(3):648-63. doi: 10.1111/j.1365-2958.2011.07841.x. Epub 2011 Sep 30.
2
Investigating local conformations of double-stranded DNA by low-energy circular dichroism of pyrrolo-cytosine.通过吡咯 - 胞嘧啶的低能圆二色性研究双链DNA的局部构象
Proc Natl Acad Sci U S A. 2005 May 17;102(20):7169-73. doi: 10.1073/pnas.0502359102. Epub 2005 May 9.
3
Low-energy circular dichroism of 2-aminopurine dinucleotide as a probe of local conformation of DNA and RNA.以2-氨基嘌呤二核苷酸的低能圆二色性作为DNA和RNA局部构象的探针
Proc Natl Acad Sci U S A. 2004 Mar 9;101(10):3426-31. doi: 10.1073/pnas.0400591101. Epub 2004 Mar 1.
4
DNA polymerase fidelity: from genetics toward a biochemical understanding.DNA聚合酶保真度：从遗传学走向生化理解
Genetics. 1998 Apr;148(4):1475-82. doi: 10.1093/genetics/148.4.1475.
5
Mechanism of 2-aminopurine mutagenesis in mouse T-lymphosarcoma cells.2-氨基嘌呤在小鼠T淋巴细胞肉瘤细胞中的诱变机制。
Mol Cell Biol. 1982 Sep;2(9):1096-103. doi: 10.1128/mcb.2.9.1096-1103.1982.
6
Kinetic measurement of 2-aminopurine X cytosine and 2-aminopurine X thymine base pairs as a test of DNA polymerase fidelity mechanisms.对2-氨基嘌呤与胞嘧啶及2-氨基嘌呤与胸腺嘧啶碱基对进行动力学测量，以此作为DNA聚合酶保真机制的一项测试。
Proc Natl Acad Sci U S A. 1982 Nov;79(21):6429-33. doi: 10.1073/pnas.79.21.6429.
7
On the molecular basis of transition mutations: frequencies of forming 2-aminopurine.cytosine and adenine.cytosine base mispairs in vitro.关于转换突变的分子基础：体外形成2-氨基嘌呤·胞嘧啶和腺嘌呤·胞嘧啶碱基错配的频率
Proc Natl Acad Sci U S A. 1981 May;78(5):2864-8. doi: 10.1073/pnas.78.5.2864.
8
Deoxyribonucleotide pools, base pairing, and sequence configuration affecting bromodeoxyuridine- and 2-aminopurine-induced mutagenesis.脱氧核苷酸库、碱基配对和序列构型对溴脱氧尿苷和2-氨基嘌呤诱导的诱变作用的影响。
Proc Natl Acad Sci U S A. 1980 Apr;77(4):1801-5. doi: 10.1073/pnas.77.4.1801.
9
Effect of proofreading and dam-instructed mismatch repair systems on N4-hydroxycytidine-induced mutagenesis.
Mol Gen Genet. 1982;186(3):411-8. doi: 10.1007/BF00729462.
10
Deoxynucleoside [1-thio]triphosphates prevent proofreading during in vitro DNA synthesis.脱氧核苷[1-硫代]三磷酸在体外DNA合成过程中阻止校对。
Proc Natl Acad Sci U S A. 1981 Nov;78(11):6734-8. doi: 10.1073/pnas.78.11.6734.
本文引用的文献
1
On the specificity of DNA polymerase.论DNA聚合酶的特异性
Proc Natl Acad Sci U S A. 1967 Mar;57(3):650-7. doi: 10.1073/pnas.57.3.650.
2
Reversal of mutant phenotypes by 5-fluorouracil: an approach to nucleotide sequences in messenger-RNA.5-氟尿嘧啶对突变表型的逆转：一种研究信使核糖核酸中核苷酸序列的方法。
Proc Natl Acad Sci U S A. 1962 Apr 15;48(4):532-46. doi: 10.1073/pnas.48.4.532.
3
Mutation as an error in base pairing.作为碱基配对错误的突变。
Biochem Biophys Res Commun. 1960 Sep;3:275-80. doi: 10.1016/0006-291x(60)90239-4.
4
A determination of mutagen specificity in bacteria using nonsense mutants of bacteriophage T4.利用噬菌体T4的无义突变体测定细菌中的诱变特异性。
J Mol Biol. 1967 Jun 28;26(3):437-47. doi: 10.1016/0022-2836(67)90314-2.
5
The mutagenic effect of hydroxyaminopurine derivatives on phage T4.羟氨基嘌呤衍生物对噬菌体T4的诱变作用。
Mutat Res. 1968 Mar-Apr;5(2):299-301. doi: 10.1016/0027-5107(68)90028-6.
6
Properties of bacteriophage T4 mutants defective in DNA polymerase.DNA聚合酶缺陷的噬菌体T4突变体的特性
Genetics. 1970 Jun;65(2):187-200. doi: 10.1093/genetics/65.2.187.
7
The influence of neighboring base pairs upon base-pair substitution mutation rates.相邻碱基对对碱基对替换突变率的影响。
Proc Natl Acad Sci U S A. 1971 Apr;68(4):773-6. doi: 10.1073/pnas.68.4.773.
8
Antimutagenic DNA polymerases of bacteriophage T4.噬菌体T4的抗诱变DNA聚合酶
Cold Spring Harb Symp Quant Biol. 1968;33:339-44. doi: 10.1101/sqb.1968.033.01.039.
9
On the role of DNA polymerase in base selection.论DNA聚合酶在碱基选择中的作用。
Cold Spring Harb Symp Quant Biol. 1966;31:693-7. doi: 10.1101/sqb.1966.031.01.088.
10
The structural gene for deoxyribonucleic acid polymerase in bacteriophages T4 and T5.噬菌体T4和T5中脱氧核糖核酸聚合酶的结构基因。
Proc Natl Acad Sci U S A. 1965 Oct;54(4):1241-8. doi: 10.1073/pnas.54.4.1241.
Zotero 插件