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膳食壳聚糖纳米颗粒可保护克氏原螯虾免受白斑综合征病毒(WSSV)感染。

Dietary chitosan nanoparticles protect crayfish Procambarus clarkii against white spot syndrome virus (WSSV) infection.

作者信息

Sun Baozhen, Quan Haizhi, Zhu Fei

机构信息

College of Animal Science and Technology, Zhejiang Agriculture and Forestry University, Hangzhou 311300, China.

Institute of Feed Science, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China.

出版信息

Fish Shellfish Immunol. 2016 Jul;54:241-6. doi: 10.1016/j.fsi.2016.04.009. Epub 2016 Apr 9.

Abstract

Chitosan nanoparticles have exhibited potential antibacterial activity or anticancer activity as their unique character. In this study, we investigated the effect of chitosan nanoparticles protect crayfish Procambarus clarkii against WSSV. Chitosan (from crab shell) nanoparticles were prepared by ultrafine milling. The physicochemical properties of the nanoparticles were determined by particle size measure, zeta potential analysis and scanning electron microscope observation. The total hemocyte count (THC), phenoloxidase (PO) and superoxide dismutase (SOD) activity were measured at days 1, 4, 9 and 12, and the survival rate was also recorded after WSSV challenge. The results showed that chitosan nanoparticles could enhance the survival rate of WSSV-challenged crayfish. And crayfish fed diets supplemented with 10 mg/g chitosan nanoparticles (65% mortality) showed a significantly higher survival rate when compared to the control group (100% mortality). The analysis of immunological parameters revealed that 10 mg/g chitosan nanoparticles showed significantly higher level of prophenoloxidase (proPO), superoxide dismutase (SOD) and total hemocyte count (THC) when compared to the control group. It was found that chitosan nanoparticles could inhibit WSSV replication in crayfish. Our results demonstrated that dietary chitosan nanoparticles effectively improve innate immunity and survival of P. clarkii challenged with WSSV.

摘要

壳聚糖纳米颗粒凭借其独特性质展现出了潜在的抗菌活性或抗癌活性。在本研究中,我们探究了壳聚糖纳米颗粒对克氏原螯虾抵御白斑综合征病毒(WSSV)的保护作用。通过超微粉碎制备了(来自蟹壳的)壳聚糖纳米颗粒。通过粒度测量、zeta电位分析和扫描电子显微镜观察来测定纳米颗粒的理化性质。在第1天、第4天、第9天和第12天测量总血细胞计数(THC)、酚氧化酶(PO)和超氧化物歧化酶(SOD)活性,并在WSSV攻毒后记录存活率。结果表明,壳聚糖纳米颗粒可以提高受WSSV攻击的克氏原螯虾的存活率。与对照组(100%死亡率)相比,喂食添加10 mg/g壳聚糖纳米颗粒饲料的克氏原螯虾(死亡率65%)显示出显著更高的存活率。免疫参数分析表明,与对照组相比,10 mg/g壳聚糖纳米颗粒组的前酚氧化酶(proPO)、超氧化物歧化酶(SOD)和总血细胞计数(THC)水平显著更高。研究发现壳聚糖纳米颗粒可以抑制WSSV在克氏原螯虾体内的复制。我们的结果表明,饲料中的壳聚糖纳米颗粒可有效提高受WSSV攻击的克氏原螯虾的先天免疫力和存活率。

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