School of Life Sciences, Shanxi University, Wucheng Road 92, Taiyuan 030006, PR China.
Fish Shellfish Immunol. 2011 Dec;31(6):1122-8. doi: 10.1016/j.fsi.2011.10.002. Epub 2011 Oct 10.
There is growing evidence that recombinant VP28 protein (rVP28) can significantly enhance immune response and disease resistance against white spot syndrome virus (WSSV) in shrimp, although the underlying mechanisms have not been entirely clarified yet. The aim of this study was to determine the effect of rVP28 on histological alterations and WSSV-induced apoptosis in crayfish Procambarus clarkii. Crayfish were fed commercial diets supplemented with different doses of HyNPV-VP28 infected pupae (rVP28-hp) for 4 weeks. Results showed that rVP28-hp may be used as a safe and effective source of medicinal proteins in aquaculture when supplemented in diet at low dose (10 g kg(-1) and 50 g kg(-1)), which could obviously reduce the percentage of apoptotic cells in stomach, gut and hepatopancreas tissues induced by the WSSV challenge and showed the relative percent survival (RPS) of 82.2% and 94.4%, respectively. But rVP28-hp would be detrimental to crayfish survival and decrease resistance to WSSV infection at the high dose (100 g kg(-1) and 200 g kg(-1)), with the cumulative mortality of up to 48.2% and 56.6% after WSSV challenge, respectively. During a 28-d feeding period, the survival rate of crayfish was only 54.5%-75.6%, and histopathological observation showed that one of the principal lesions was serious cell swelling, vacuolar degeneration and necrosis in hepatopancreatic epithelia and myocardial cells. These results suggested that rVP28-hp can influence the immune functions of crayfish in a dose-dependent manner, and the rVP28-hp at the dose of 50 g kg(-1) was recommended to prevent WSSV in crayfish culture.
越来越多的证据表明，重组 VP28 蛋白（rVP28）可显著增强虾对白斑综合征病毒（WSSV）的免疫反应和抗病能力，尽管其潜在机制尚未完全阐明。本研究旨在确定 rVP28 对克氏原螯虾组织学变化和 WSSV 诱导的细胞凋亡的影响。克氏原螯虾投喂添加不同剂量的携带 HyNPV-VP28 感染蛹的商业饲料（rVP28-hp）4 周。结果表明，rVP28-hp 可作为一种安全有效的水产养殖药用蛋白来源，当低剂量（10 g kg(-1) 和 50 g kg(-1)）添加到饲料中时，可明显降低 WSSV 攻毒引起的胃、肠和肝胰腺组织中凋亡细胞的比例，相对存活率（RPS）分别为 82.2%和 94.4%。但 rVP28-hp 会对克氏原螯虾的存活造成损害，并降低其对 WSSV 感染的抵抗力，高剂量（100 g kg(-1) 和 200 g kg(-1)）时，WSSV 攻毒后的累积死亡率分别高达 48.2%和 56.6%。在 28 天的投喂期内，克氏原螯虾的存活率仅为 54.5%-75.6%，组织病理学观察表明，主要病变之一是肝胰腺上皮细胞和心肌细胞严重细胞肿胀、空泡变性和坏死。这些结果表明，rVP28-hp 可剂量依赖性地影响克氏原螯虾的免疫功能，建议在克氏原螯虾养殖中以 50 g kg(-1) 的剂量使用 rVP28-hp 来预防 WSSV。
