Zhou Sheng, Nikolausz Marcell, Zhang Jining, Riya Shohei, Terada Akihiko, Hosomi Masaaki
Eco-environmental Protection Research Institute, Shanghai Academy of Agricultural Sciences, No. 1000 Jinqi Road, Shanghai 201403, China.
Department of Environmental Microbiology, UFZ - Helmholtz Centre for Environmental Research, Permoserstraße 15, 04318 Leipzig, Germany.
J Biosci Bioeng. 2016 Sep;122(3):334-40. doi: 10.1016/j.jbiosc.2016.02.012. Epub 2016 Apr 9.
The effect of pig manure mixed with rice straw on methane yield and the microbial community involved in a thermophilic (55°C) anaerobic digestion process was investigated. Three substrates composed of mixed pig manure and rice straw at different ratios (95:5; 78:22 and 65:35 w/w, which resulted in C/N ratios of 10:1, 20:1 and 30:1) were used for the experiment. The substrate type had a major influence on the total bacterial community, while the methanogens were less affected. The members of the class Clostridia (phylum Firmicutes) were predominant regardless of mixture ratio (C/N ratio), but at species level there was a major difference between the low and high C/N ratio samples. The hydrogenotrophic methanogenic genus of Methanothermobacter was predominant in all samples but higher C/N ratio sequences affiliated to the genus Methanosarcina were also detected. The appearance of Methanosarcina sp. is most likely due to the less inhibition of ammonia during the anaerobic digestion.
研究了猪粪与稻草混合对嗜热（55°C）厌氧消化过程中甲烷产量及相关微生物群落的影响。使用了三种由不同比例（95:5；78:22和65:35 w/w，其C/N比分别为10:1、20:1和30:1）的猪粪与稻草混合而成的底物进行实验。底物类型对总细菌群落有主要影响，而产甲烷菌受影响较小。无论混合比例（C/N比）如何，梭菌纲（厚壁菌门）的成员均占主导地位，但在物种水平上，低C/N比和高C/N比样本之间存在重大差异。嗜氢产甲烷菌属的嗜热栖热甲烷杆菌在所有样本中均占主导地位，但也检测到了与甲烷八叠球菌属相关的高C/N比序列。甲烷八叠球菌属的出现很可能是由于厌氧消化过程中氨的抑制作用较小。
