用于弗格森图分析的BASIC程序，使用个人计算机：在连续缓冲液中的凝胶电泳应用。

Program in BASIC for Ferguson plot analysis, using a personal computer: application to gel electrophoresis in a continuous buffer.

作者信息

Gombocz E, Chrambach A

机构信息

Section on Macromolecular Analysis, National Institute of Child Health and Human Development, Bethesda, MD 20892.

出版信息

Electrophoresis. 1989 Mar;10(3):199-214. doi: 10.1002/elps.1150100309.

DOI:10.1002/elps.1150100309

PMID:2707243

Abstract

A program in BASIC suitable for personal computers is described which is applicable to gel electrophoresis conducted in a single (continuous) buffer. The curve fitting is to a polynomial function, allowing for an objective selection of the most appropriate curve type and order--linear, convex or concave--in the particular application. Results do not differ significantly from previous programs for evaluation of linear Ferguson plots or of curve fitting to an exponential function for evaluating convex plots, executed on mainframe computers such as the DEC-10 (Digital) and IBM 370 computers. Thus, the program combines original versatility with, for the first time, the possibility for widespread application of Ferguson plot analysis on personal computers.

摘要

本文描述了一个适用于个人计算机的BASIC程序，该程序适用于在单一（连续）缓冲液中进行的凝胶电泳。曲线拟合采用多项式函数，可在特定应用中客观选择最合适的曲线类型和阶数——线性、凸形或凹形。对于在大型计算机（如DEC-10（数字）和IBM 370计算机）上执行的用于评估线性弗格森图或用于评估凸形图的指数函数曲线拟合的先前程序，结果没有显著差异。因此，该程序首次将原始的通用性与在个人计算机上广泛应用弗格森图分析的可能性结合在一起。