Pan Yanyan, Wu Yuanyuan, Ji Jun, Cai Hongjiao, Wang Heshuang, Jiang Yifan, Sang Limin, Yang Jin, Gao Yanyan, Liu Ying, Yin Liangwei, Zhang L I
Department of Central Laboratory, Dalian Municipal Central Hospital, Dalian, Liaoning 116033, P.R. China.
Department of Cell Biological Treatment, Dalian Municipal Central Hospital, Dalian, Liaoning 116033, P.R. China.
Oncol Lett. 2016 Apr;11(4):2827-2834. doi: 10.3892/ol.2016.4284. Epub 2016 Feb 29.
Cytokine-induced killer (CIK) cells have been used as adoptive immunotherapy in cancer. The present study evaluated the effect of CIK cells on immune function in patients with lung cancer. Patients were divided into three groups, according to the treatment received prior to CIK cell treatment: CIK group (no prior treatment), Che-Sur group (prior chemotherapy and surgery) and Che-Rad group (prior chemotherapy and radiotherapy). Following treatment, the average percentage of cluster of differentiation (CD)3CD4, CD3, natural killer (NK) and NKT cells in peripheral blood was significantly higher than that prior to CIK treatment in the Che-Sur and CIK groups, and the levels of interferon-γ in serum were significantly higher than those prior to CIK treatment in the Che-Sur and CIK groups. On the contrary, the levels of interleukin-10 had decreased in these groups following CIK treatment. Subsequently, patients were divided into three groups according to the percentage of CD3CD56 CIK cells that were administered to the patients. The number of NK and NKT cells increased with increasing number of CD3CD56 cells. The patients in the CIK and Che-Sur groups were the most benefited ones following CIK treatment, contrarily to those in the Che-Rad group, since the increase in the number of CD3CD56 CIK cells in the aforementioned patients enhanced the number of NK cells, which exhibit antitumor activity.
细胞因子诱导的杀伤细胞（CIK）已被用作癌症的过继性免疫疗法。本研究评估了CIK细胞对肺癌患者免疫功能的影响。根据CIK细胞治疗前接受的治疗，将患者分为三组：CIK组（无先前治疗）、化疗-手术组（先前接受化疗和手术）和化疗-放疗组（先前接受化疗和放疗）。治疗后，化疗-手术组和CIK组外周血中分化簇（CD）3CD4、CD3、自然杀伤（NK）和NKT细胞的平均百分比显著高于CIK治疗前，血清中干扰素-γ水平显著高于化疗-手术组和CIK组CIK治疗前。相反，这些组在CIK治疗后白细胞介素-10水平有所下降。随后，根据给予患者的CD3CD56 CIK细胞百分比将患者分为三组。NK和NKT细胞的数量随着CD3CD56细胞数量的增加而增加。CIK组和化疗-手术组的患者在CIK治疗后受益最大，与化疗-放疗组的患者相反，因为上述患者中CD3CD56 CIK细胞数量的增加增强了具有抗肿瘤活性的NK细胞的数量。
