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从牛瘤胃分离的牛链球菌对坦桑尼亚青草青贮饲料发酵特性和营养价值的影响

Effects of Streptococcus bovis Isolated from Bovine Rumen on the Fermentation Characteristics and Nutritive Value of Tanzania Grass Silage.

作者信息

Zanine Anderson de Moura, Bonelli Emerson Alencar, de Souza Alexandre Lima, Ferreira Daniele de Jesus, Santos Edson Mauro, Ribeiro Marinaldo Divino, Geron Luiz Juliano Valério, Pinho Ricardo Martins Araujo

机构信息

Department of Animal Science, Federal University of Maranhão, 65500-000 Chapadinha, MA, Brazil.

Department of Animal Science, Federal University of Mato Grosso, 78735-901 Rondonópolis, MT, Brazil.

出版信息

ScientificWorldJournal. 2016;2016:8517698. doi: 10.1155/2016/8517698. Epub 2016 Mar 17.

Abstract

This study aimed to evaluate the effects of Streptococcus bovis on the fermentation characteristics and nutritive value of Tanzania grass silage. Tanzania grass was chopped and left untreated (U) or treated with Streptococcus bovis JB1 at 1 × 10(6) colony-forming units per gram (cfu/g) of fresh forage or Streptococcus bovis HC5 at 1 × 10(6) cfu/g of fresh forage and packed into sixtuplicate laboratory silos. The largest number of enterobacteria, molds and yeast (M&Y) occurred in untreated silages and the smallest populations of enterobacteria and M&Y and the largest numbers of lactic acid bacteria (LAB), at 9.81 and 9.87 log cfu/g, were observed in Streptococcus bovis JB1 and HC5, respectively (P < 0.05). Silages treated with JB1 and HC5 had lower (P < 0.05) silage pHs and concentrations of ammoniacal nitrogen (NH3-N) than untreated silages. The application of Streptococcus bovis JB1 and HC5 resulted in fewer losses through gases and effluents (P < 0.05), which resulted in greater dry matter recovery (DMR) and crude protein recovery (CPR) (P < 0.05). Streptococcus bovis JB1 and HC5 improved the fermentative profile and increased the concentration of crude protein and DMR and CPR in Tanzania grass silage.

摘要

本研究旨在评估牛链球菌对坦桑尼亚草青贮饲料发酵特性和营养价值的影响。将坦桑尼亚草切碎,不进行处理(U),或用每克新鲜草料含1×10⁶ 菌落形成单位(cfu/g)的牛链球菌JB1或每克新鲜草料含1×10⁶ cfu/g的牛链球菌HC5处理,然后装入六个重复的实验室青贮窖中。未处理的青贮饲料中肠杆菌、霉菌和酵母菌(M&Y)数量最多,而在牛链球菌JB1和HC5处理的青贮饲料中,肠杆菌和M&Y数量最少,乳酸菌(LAB)数量最多,分别为9.81和9.87 log cfu/g(P < 0.05)。用JB1和HC5处理的青贮饲料的青贮pH值和氨态氮(NH₃-N)浓度低于未处理的青贮饲料(P < 0.05)。应用牛链球菌JB1和HC5减少了气体和流出物造成的损失(P < 0.05),从而提高了干物质回收率(DMR)和粗蛋白回收率(CPR)(P < 0.05)。牛链球菌JB1和HC5改善了发酵情况,提高了坦桑尼亚草青贮饲料中粗蛋白的浓度、DMR和CPR。

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