Song Chengwu, Yu Qingsong, Li Xiaohua, Jin Shuna, Li Sen, Zhang Yang, Jia Shuailong, Chen Cheng, Xiang Yi, Jiang Hongliang
Tongji School of Pharmacy, Huazhong Univ. of Science and Technology, 13 Hangkong Road, Wuhan, Hubei, China.
Dept. of Otolaryngology, Union Hospital, Tongji Medical College, Huazhong Univ. of Science and Technology, 13 Hangkong Road, Wuhan, Hubei, China.
J Food Sci. 2016 May;81(5):H1313-9. doi: 10.1111/1750-3841.13299. Epub 2016 Apr 13.
Kuding tea are used as a traditional tea material and widely consumed in China. In this study, total saponins (TS) from water extract of Kuding tea was prepared by D101 macroporous resins and analyzed by UPLC-QTOF-MS/MS. Then the hypolipidemic effect of TS extract was investigated in high-fat diet-induced hyperlipidemic mice. For comprehensive identification or characterization of saponins in TS extract, 3 major saponins of Kudinoside A, Kudinoside F, and Kudinoside D were isolated and used as standards to investigate the MS/MS fragmentation pattern. As a result, 52 saponins were identified or characterized in TS extract from Kuding tea. In addition, the increased levels of mice serum TC, LDL-C, HDL-C, and atherogenic index (AI) were significantly reduced after the treatment of TS extract. Also, the liver protective effect of TS extract was obviously judged from the photographs stained with oil red-O staining. Meanwhile, TS extract significantly upregulated the expression of hepatic scavenger receptors including SR-AI, SR-BI, and CD36. Therefore, it is reasonable to assume that the overexpression of hepatic scavenger receptors was involved in the hypolipidemic effect of Kuding tea on the high-fat diet-induced hyperlipidemic mice. The TS extract could influence these scavenger receptors, and this could be the potential mechanism of TS extract from Kuding tea in the treatment of lipid disorders. These results give the evidence that the saponins in Kuding tea could provide benefits in managing hypercholesterolemia and may be a good candidate for development as a functional food and nutraceutical.
苦丁茶作为一种传统茶原料在中国被广泛饮用。在本研究中，采用D101大孔树脂制备苦丁茶水提取物中的总皂苷（TS），并通过超高效液相色谱-四极杆飞行时间串联质谱（UPLC-QTOF-MS/MS）进行分析。然后在高脂饮食诱导的高脂血症小鼠中研究TS提取物的降血脂作用。为了全面鉴定或表征TS提取物中的皂苷，分离出3种主要皂苷苦丁苷A、苦丁苷F和苦丁苷D作为标准品，研究其MS/MS裂解模式。结果，在苦丁茶TS提取物中鉴定或表征了52种皂苷。此外，用TS提取物处理后，小鼠血清总胆固醇（TC）、低密度脂蛋白胆固醇（LDL-C）、高密度脂蛋白胆固醇（HDL-C）水平及动脉粥样硬化指数（AI）的升高均显著降低。同时，从油红O染色照片可明显判断TS提取物对肝脏有保护作用。此外，TS提取物显著上调了包括清道夫受体AⅠ（SR-AⅠ）、清道夫受体BⅠ（SR-BⅠ）和CD36在内的肝脏清道夫受体的表达。因此，可以合理推测肝脏清道夫受体的过表达参与了苦丁茶对高脂饮食诱导的高脂血症小鼠的降血脂作用。TS提取物可能影响这些清道夫受体，这可能是苦丁茶TS提取物治疗脂质紊乱的潜在机制。这些结果证明苦丁茶中的皂苷在管理高胆固醇血症方面可能有益，并且可能是开发功能性食品和营养保健品的良好候选物。
