Adams R L, So C L
Department of Biochemistry, University of Glasgow, Scotland.
FEBS Lett. 1989 Mar 27;246(1-2):54-6. doi: 10.1016/0014-5793(89)80252-2.
We have analysed the 5-methylcytosine content of hen erythrocyte DNA and found it to be lower than that of DNA from other chick tissues analysed. Erythrocyte DNA is also a better substrate for DNA methylase having a five-fold lower Km than DNA from white blood cells. This is probably because it contains a large number of hemimethylated sites. Thus the inverse correlation between methylation and gene expression does not apply to the chick red blood cell.
我们分析了母鸡红细胞DNA的5-甲基胞嘧啶含量，发现其低于所分析的其他鸡组织的DNA。红细胞DNA也是DNA甲基化酶的更好底物，其Km值比白细胞的DNA低五倍。这可能是因为它含有大量半甲基化位点。因此，甲基化与基因表达之间的负相关不适用于鸡的红细胞。
