Chen Ting, Ren Chunhua, Wang Yanhong, Luo Peng, Jiang Xiao, Huang Wen, Chen Chang, Hu Chaoqun
CAS Key Laboratory of Tropical Marine Bio-resources and Ecology (LMB), Guangdong Provincial Key Laboratory of Applied Marine Biology (LAMB), South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, China; South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, Guangzhou, China.
CAS Key Laboratory of Tropical Marine Bio-resources and Ecology (LMB), Guangdong Provincial Key Laboratory of Applied Marine Biology (LAMB), South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, China.
Fish Shellfish Immunol. 2016 Jul;54:197-203. doi: 10.1016/j.fsi.2016.04.008. Epub 2016 Apr 10.
The full-length cDNA coding for a novel invertebrate (i-type) lysozyme was identified in Pacific white shrimp (Litopenaeus vannamei). The newly obtained L. vannamei lysozyme is similar to the Penaeus monodon i-type lysozyme 2, but it is distant from the known L. vannamei c-type lysozyme and i-type lysozyme 1 in protein sequence; therefore, it was defined as L. vannamei i-type lysozyme 2 (lyz-i2). Expression of L. vannamei lyz-i2 transcripts were ubiquitously detected in all tissues we selected, with the highest abundance observed in the hemolymph. Challenge with Vibrio harveyi might elicit L. vannamei lyz-i2 mRNA expression in the hepatopancreas, intestine, muscle, gill and hemolymph. In the themolymph, specifically, the stimulatory effects of Vibrio and lipopolysaccharide (LPS) on lyz-i2 transcript levels were durable and transient, respectively; while Polyinosinic:polycytidylic acid [Poly (I:C)] treatment did not affect lyz-i2 expression. L. vannamei lyz-i2 recombinant protein was generated in an Escherichia coli system. By lysoplate and turbidimetric assays, the L. vannamei lyz-i2 recombinant protein showed a broad spectrum of antimicrobial properties with high activities against Micrococcaceae lysodeikticus and various Vibrio species and relatively low activity against E. coli. In conclusion, L. vannamei lyz-i2 might be a potent antibacterial protein with a role in innate immunity in Penaeid shrimp.
在南美白对虾（凡纳滨对虾）中鉴定出了编码一种新型无脊椎动物（i型）溶菌酶的全长cDNA。新获得的凡纳滨对虾溶菌酶与斑节对虾i型溶菌酶2相似，但在蛋白质序列上与已知的凡纳滨对虾c型溶菌酶和i型溶菌酶1距离较远；因此，它被定义为凡纳滨对虾i型溶菌酶2（lyz-i2）。在我们选择的所有组织中均普遍检测到凡纳滨对虾lyz-i2转录本的表达，其中在血淋巴中观察到的丰度最高。用哈维氏弧菌进行攻毒可能会引发凡纳滨对虾肝胰腺、肠道、肌肉、鳃和血淋巴中lyz-i2 mRNA的表达。具体而言，在血淋巴中，弧菌和脂多糖（LPS）对lyz-i2转录水平的刺激作用分别是持久的和短暂的；而聚肌苷酸:聚胞苷酸[Poly（I:C）]处理不影响lyz-i2的表达。凡纳滨对虾lyz-i2重组蛋白在大肠杆菌系统中产生。通过溶菌平板法和比浊法测定，凡纳滨对虾lyz-i2重组蛋白表现出广谱抗菌特性，对溶壁微球菌和各种弧菌具有高活性，而对大肠杆菌的活性相对较低。总之，凡纳滨对虾lyz-i2可能是一种在对虾先天免疫中起作用的强效抗菌蛋白。
