人血浆纤连蛋白胰凝乳蛋白酶胶原结合片段的去糖基化不会改变其与变性胶原的亲和力。
Deglycosylation of the chymotryptic collagen-binding fragment of human plasma fibronectin does not modify its affinity to denatured collagen.
作者信息
Delannoy P, Montreuil J
机构信息
Laboratoire de Chimie Biologique, Université des Sciences et Techniques de Lille Villenuve d'Ascq, France.
出版信息
FEBS Lett. 1989 Apr 10;247(1):25-7. doi: 10.1016/0014-5793(89)81232-3.
PMID:2707448
Abstract
N-Glycanase deglycosylation of purified 44 kDa chymotryptic collagen-binding domain from human plasma fibronectin does not significantly modify its behavior on gelatin affinity chromatography. This indicates that carbohydrates do not play any role in the binding affinity of fibronectin to collagen. The influence of changes in glycosylation on the biological functions of fibronectin is discussed.
摘要
对人血浆纤连蛋白中纯化的44 kDa胰凝乳蛋白酶胶原结合结构域进行N-聚糖酶去糖基化处理，并不会显著改变其在明胶亲和色谱上的行为。这表明碳水化合物在纤连蛋白与胶原的结合亲和力中不发挥任何作用。文中讨论了糖基化变化对纤连蛋白生物学功能的影响。
相似文献
1
Deglycosylation of the chymotryptic collagen-binding fragment of human plasma fibronectin does not modify its affinity to denatured collagen.人血浆纤连蛋白胰凝乳蛋白酶胶原结合片段的去糖基化不会改变其与变性胶原的亲和力。
FEBS Lett. 1989 Apr 10;247(1):25-7. doi: 10.1016/0014-5793(89)81232-3.
2
Human placental (fetal) fibronectin: increased glycosylation and higher protease resistance than plasma fibronectin. Presence of polylactosamine glycopeptides and properties of a 44-kilodalton chymotryptic collagen-binding domain: difference from human plasma fibronectin.人胎盘（胎儿）纤连蛋白：糖基化增加，且比血浆纤连蛋白具有更高的蛋白酶抗性。存在多乳糖胺糖肽以及44千道尔顿胰凝乳蛋白酶敏感的胶原结合结构域的特性：与人血浆纤连蛋白的差异。
J Biol Chem. 1984 Mar 25;259(6):3962-70.
3
Polylactosamine glycosylation on human fetal placental fibronectin weakens the binding affinity of fibronectin to gelatin.
J Biol Chem. 1985 Apr 10;260(7):4041-5.
4
Isolation of an actin-binding fragment of fibronectin.纤连蛋白肌动蛋白结合片段的分离
Biochem J. 1981 Feb 1;193(2):615-20. doi: 10.1042/bj1930615.
5
Use of biospecific interactions of collagen, fibronectin and their fragments in affinity chromatography.
J Chromatogr. 1992 Jun 10;577(2):267-73. doi: 10.1016/0378-4347(92)80247-n.
6
Affinity chromatography of collagen on collagen-binding fragments of fibronectin.纤连蛋白的胶原结合片段上胶原蛋白的亲和层析
Coll Relat Res. 1981 Nov;1(6):505-16. doi: 10.1016/s0174-173x(81)80032-5.
7
Cryptic chemotactic activity of fibronectin for human monocytes resides in the 120-kDa fibroblastic cell-binding fragment.纤连蛋白对人单核细胞的隐蔽趋化活性存在于120 kDa的成纤维细胞结合片段中。
J Biol Chem. 1988 Aug 25;263(24):12115-23.
8
Affinity of human erythrocyte transglutaminase for a 42-kDa gelatin-binding fragment of human plasma fibronectin.人红细胞转谷氨酰胺酶对人血浆纤连蛋白42 kDa明胶结合片段的亲和力。
Proc Natl Acad Sci U S A. 1993 Apr 15;90(8):3152-6. doi: 10.1073/pnas.90.8.3152.
9
Functional methionines in the collagen/gelatin binding domain of plasma fibronectin: effects of chemical modification by chloramine T.血浆纤连蛋白胶原/明胶结合域中的功能性甲硫氨酸：氯胺T化学修饰的影响
Biochemistry. 1993 Aug 17;32(32):8168-78. doi: 10.1021/bi00083a017.
10
Purification of Human Plasma/Cellular Fibronectin and Fibronectin Fragments.人血浆/细胞纤连蛋白及纤连蛋白片段的纯化
Methods Mol Biol. 2017;1627:309-324. doi: 10.1007/978-1-4939-7113-8_20.
Zotero 插件