体外重组核不均一核糖核蛋白颗粒
In vitro reconstitution of hnRNP particles.
作者信息
Schenkel J, Kloetzel P M
机构信息
University of Heidelberg Medical School, FRG.
出版信息
FEBS Lett. 1989 Apr 10;247(1):51-4. doi: 10.1016/0014-5793(89)81238-4.
The assembly of hnRNP-like particles was studied by in vitro reconstitution, UV-crosslinking and CsCl-equilibrium centrifugation. Using total nuclear protein and RNA extracts from HeLa cells for RNP reconstitution, RNP particles sedimenting with the same buoyant density of p = 1.4 g/cm3 as 'native' 40 S core hnRNPs were obtained. Under the stringent reconstitution conditions used, hnRNP complexes containing only the Cl-core hnRNP protein could be identified.
通过体外重组、紫外线交联和氯化铯平衡离心法研究了类hnRNP颗粒的组装。使用来自HeLa细胞的总核蛋白和RNA提取物进行RNP重组，获得了与“天然”40S核心hnRNPs具有相同浮力密度（p = 1.4 g/cm3）的RNP颗粒。在所使用的严格重组条件下，可以鉴定出仅含有Cl核心hnRNP蛋白的hnRNP复合物。
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