Bivona Augusto E, Cerny Natacha, Alberti Andrés Sánchez, Cazorla Silvia I, Malchiodi Emilio L
Cátedra de Inmunología and Instituto de Estudios de la Inmunidad Humoral (IDEHU), CONICET-UBA, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 956 4to P, Buenos Aires, C1113AAD, Argentina.
Instituto de Microbiología y Parasitología Médica, IMPaM (UBA-CONICET) y Departamento de Microbiología, Parasitología e Inmunología, Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires, Argentina.
Methods Mol Biol. 2016;1404:683-695. doi: 10.1007/978-1-4939-3389-1_44.
Chagas disease is an important neglected disease affecting thousands of people in the Americas. Novel strategies for prophylactic and therapeutic vaccines against the etiological agent, the intracellular protozoan Trypanosoma cruzi, are urgently needed. Vaccines based on attenuated virus and bacteria as a foreign DNA delivery system represent a strong advantage over naked DNA-based vaccines. Here we describe the use of attenuated Salmonella carrying a eukaryotic expression plasmid encoding a T. cruzi antigen. The main advantages of the methodology are the oral administration of the Salmonella-based vaccine and the induction of a strong humoral and cell-mediated immune response at both mucosal and systemic level, favored by the adjuvant effect elicited by the bacteria pathogen-associated molecular patterns.
恰加斯病是一种重要的被忽视疾病，影响着美洲成千上万的人。迫切需要针对病原体——细胞内原生动物克氏锥虫的预防性和治疗性疫苗的新策略。基于减毒病毒和细菌作为外源DNA递送系统的疫苗比基于裸DNA的疫苗具有显著优势。在此，我们描述了携带编码克氏锥虫抗原的真核表达质粒的减毒沙门氏菌的应用。该方法的主要优点是基于沙门氏菌的疫苗可口服给药，并且在粘膜和全身水平均可诱导强烈的体液免疫和细胞介导免疫反应，这得益于细菌病原体相关分子模式引发的佐剂效应。
