Miranzadeh-Mahabadi Hajar, Emadi-Baygi Modjtaba, Nikpour Parvaneh, Kelishadi Roya
Department of Genetics, School of Basic Sciences, Shahrekord University, Shahrekord, Iran; Child Growth and Development Research Center, Research Institute for Primordial Prevention of Non-communicable Disease, Isfahan University of Medical Sciences, Isfahan, Iran.
Department of Genetics, School of Basic Sciences, Shahrekord University, Shahrekord, Iran; Research Institute of Biotechnology, Shahrekord University, Shahrekord, Iran.
Int J Prev Med. 2016 Feb 23;7:41. doi: 10.4103/2008-7802.177314. eCollection 2016.
Metabolic syndrome (MetS) is a prevalent disorder in pediatric age groups, described by a combination of genetic and environmental factors. Sterol regulatory element-binding transcription factor 1 (SREBF-1) induces the expression of a family of genes involved in fatty acid synthesis. Moreover, dysregulation of miR-33b, which is located within the intron 17 of the SREBF-1 gene, disrupts fatty acid oxidation and insulin signaling, thus leading to MetS. The aim of the present study was to investigate the association between SREBF-1 rs8066560 polymorphism and MetS in Iranian children and adolescents.
This study includes 100 MetS and 100 normal individuals aged 9-19 years. Anthropological and biochemical indexes were measured. The -1099G > A polymorphism was genotyped by TaqMan real-time polymerase chain reaction.
Significant differences were observed in anthropometric measurements and lipid profiles between MetS and normal children. There were no differences in the genotype frequencies or allele distribution for -1099G > A polymorphism between MetS and control groups. High-density lipoprotein cholesterol levels were significantly higher in the MetS GG group than in the A allele carrier group. The genotype AA controls had significantly increased cholesterol and low-density lipoprotein cholesterol levels than AG genotypes. By logistic regression using different genetic models, no significant association was observed between SREBF-1 rs8066560 polymorphism and the risk of MetS.
We conclude that the -1099G > A variant on SREBF-1 gene associated with serum lipid profiles, however, it may not be a major risk factor for the MetS in Iranian children and adolescents.
代谢综合征（MetS）在儿童年龄组中是一种普遍存在的疾病，由遗传和环境因素共同作用所致。固醇调节元件结合转录因子1（SREBF-1）可诱导参与脂肪酸合成的一系列基因的表达。此外，位于SREBF-1基因第17内含子中的miR-33b失调会破坏脂肪酸氧化和胰岛素信号传导，从而导致代谢综合征。本研究的目的是调查伊朗儿童和青少年中SREBF-1 rs8066560多态性与代谢综合征之间的关联。
本研究纳入了100名9至19岁的代谢综合征患者和100名正常个体。测量了人体学和生化指标。采用TaqMan实时聚合酶链反应对-1099G>A多态性进行基因分型。
代谢综合征患儿与正常儿童在人体测量和血脂谱方面存在显著差异。代谢综合征组与对照组之间-1099G>A多态性的基因型频率或等位基因分布没有差异。代谢综合征GG组的高密度脂蛋白胆固醇水平显著高于A等位基因携带者组。基因型为AA的对照组的胆固醇和低密度脂蛋白胆固醇水平比AG基因型显著升高。通过使用不同遗传模型的逻辑回归分析，未观察到SREBF-1 rs8066560多态性与代谢综合征风险之间存在显著关联。
我们得出结论，SREBF-1基因上的-1099G>A变异与血清脂质谱相关，然而，它可能不是伊朗儿童和青少年代谢综合征的主要危险因素。
