Hu Xiaolong, Jiang Yue, Gong Yongchang, Zhu Min, Zhu Liyuan, Chen Fei, Liang Zi, Kuang Sulan, Zar Mian Sahib, Kumar Dhiraj, Cao Guangli, Xue Renyu, Gong Chengliang
School of Biology & Basic Medical Science, Soochow University, Suzhou 215123, China; National Engineering Laboratory for Modern Silk, Soochow University, Suzhou 215123, China.
School of Biology & Basic Medical Science, Soochow University, Suzhou 215123, China.
Mol Immunol. 2016 May;73:122-9. doi: 10.1016/j.molimm.2016.04.004. Epub 2016 Apr 11.
Transforming growth factor (TGF)-β superfamily members inhibit Bombyx mori nucleohedrovirus (BmNPV) multiplication in silkworm are not determined. In this study, we first found that BmNPV RNA transcription and protein expression level were regulated by TGF-β members, Decapentaplegic (Bmdpp) and Dawdle (Bmdaw) in the domesticated silkworm, B. mori and silkworm ovary-derived cells. Furthermore, subcellular localization showed that Bmdpp and Bmdaw were mainly presented in cytomembrane of the cultured BmN cells. Tissues expression pattern analysis found that the highest expression levels of Bmdpp and Bmdaw genes were in the hemocyte of fifth instar larvae. During the immune response, the expression level of Bmdpp gene was elevated and Bmdaw gene was declined in BmNPV infected BmN cells and silkworm. The multiplication of BmNPV was inhibited by overexpression of Bmdpp and Bmdaw genes in BmN cells. RNA interference experiments found that the multiplication of BmNPV was raised with specific siRNAs of Bmdpp and Bmdaw genes in BmN cells. The antiviral immune pathways were not significantly regulated by the TGF-β superfamily members. Taken together, these findings provided a clue to understand the function of Bmdpp and Bmdaw gene in response to the BmNPV infection in silkworm.
转化生长因子（TGF）-β超家族成员对家蚕核型多角体病毒（BmNPV）在蚕体内增殖的抑制作用尚未明确。在本研究中，我们首次发现，在驯化的家蚕和家蚕卵巢来源的细胞中，BmNPV的RNA转录和蛋白质表达水平受TGF-β成员，即果蝇的Decapentaplegic（Bmdpp）和Dawdle（Bmdaw）调控。此外，亚细胞定位显示，Bmdpp和Bmdaw主要存在于培养的BmN细胞的细胞膜中。组织表达模式分析发现，Bmdpp和Bmdaw基因在五龄幼虫的血细胞中表达水平最高。在免疫反应过程中，BmNPV感染的BmN细胞和蚕中，Bmdpp基因的表达水平升高，而Bmdaw基因的表达水平下降。Bmdpp和Bmdaw基因在BmN细胞中的过表达抑制了BmNPV的增殖。RNA干扰实验发现，BmN细胞中针对Bmdpp和Bmdaw基因的特异性小干扰RNA（siRNAs）使BmNPV的增殖增加。TGF-β超家族成员对抗病毒免疫途径没有显著调控作用。综上所述，这些发现为理解Bmdpp和Bmdaw基因在蚕应对BmNPV感染中的功能提供了线索。
