Alagna Fiammetta, Cirilli Marco, Galla Giulio, Carbone Fabrizio, Daddiego Loretta, Facella Paolo, Lopez Loredana, Colao Chiara, Mariotti Roberto, Cultrera Nicolò, Rossi Martina, Barcaccia Gianni, Baldoni Luciana, Muleo Rosario, Perrotta Gaetano
Institute of Biosciences and Bioresources, National Research Council, Perugia, Italy.
Laboratory of Molecular Ecophysiology and Biotechnology of Woody Plants, Department of Agricultural and Forestry Science, University of Tuscia, Viterbo, Italy.
PLoS One. 2016 Apr 14;11(4):e0152943. doi: 10.1371/journal.pone.0152943. eCollection 2016.
The identification and characterization of transcripts involved in flower organ development, plant reproduction and metabolism represent key steps in plant phenotypic and physiological pathways, and may generate high-quality transcript variants useful for the development of functional markers. This study was aimed at obtaining an extensive characterization of the olive flower transcripts, by providing sound information on the candidate MADS-box genes related to the ABC model of flower development and on the putative genetic and molecular determinants of ovary abortion and pollen-pistil interaction. The overall sequence data, obtained by pyrosequencing of four cDNA libraries from flowers at different developmental stages of three olive varieties with distinct reproductive features (Leccino, Frantoio and Dolce Agogia), included approximately 465,000 ESTs, which gave rise to more than 14,600 contigs and approximately 92,000 singletons. As many as 56,700 unigenes were successfully annotated and provided gene ontology insights into the structural organization and putative molecular function of sequenced transcripts and deduced proteins in the context of their corresponding biological processes. Differentially expressed genes with potential regulatory roles in biosynthetic pathways and metabolic networks during flower development were identified. The gene expression studies allowed us to select the candidate genes that play well-known molecular functions in a number of biosynthetic pathways and specific biological processes that affect olive reproduction. A sound understanding of gene functions and regulatory networks that characterize the olive flower is provided.
鉴定和表征参与花器官发育、植物繁殖和代谢的转录本是植物表型和生理途径中的关键步骤，并且可能产生有助于开发功能标记的高质量转录本变体。本研究旨在通过提供与花发育ABC模型相关的候选MADS-box基因以及子房败育和花粉-雌蕊相互作用的推定遗传和分子决定因素的可靠信息，对橄榄花转录本进行广泛表征。通过对具有不同生殖特征的三个橄榄品种（莱奇诺、佛朗托io和多尔切阿戈贾）不同发育阶段的花的四个cDNA文库进行焦磷酸测序获得的总体序列数据，包括约465,000个EST，产生了超过14,600个重叠群和约92,000个单拷贝序列。多达56,700个单基因被成功注释，并在相应生物过程的背景下，对测序转录本和推导蛋白质的结构组织和推定分子功能提供了基因本体论见解。鉴定了在花发育过程中在生物合成途径和代谢网络中具有潜在调节作用的差异表达基因。基因表达研究使我们能够选择在许多生物合成途径和影响橄榄繁殖的特定生物过程中发挥众所周知分子功能的候选基因。提供了对表征橄榄花的基因功能和调控网络的深入理解。
