氯化钙激活的血小板浓缩物制备的临床级优质富血小板血浆释放物（PRP-R/SRGF）可促进间充质基质细胞的扩增。
Clinical-grade quality platelet-rich plasma releasate (PRP-R/SRGF) from CaCl2 -activated platelet concentrates promoted expansion of mesenchymal stromal cells.
作者信息
Borghese C, Agostini F, Durante C, Colombatti A, Mazzucato M, Aldinucci D
机构信息
Experimental Oncology 2, C.R.O. National Cancer Institute-IRCCS Aviano, Aviano, Italy.
Unit of Stem Cells Collection and Processing Unit, CRO Aviano, National Cancer Institute-IRCCS Aviano, Aviano, Italy.
出版信息
Vox Sang. 2016 Aug;111(2):197-205. doi: 10.1111/vox.12405. Epub 2016 Apr 14.
BACKGROUND AND OBJECTIVES
The aim of our study was to test a platelet-rich plasma releasate (PRP-R/SRGF) from CaCl2 -activated platelets as a source of growth factors for the expansion of mesenchymal stromal cells (MSCs). PRP-R/SRGF, obtained with a low-cost procedure, is characterized by a reduced variability of growth factor release.
MATERIALS AND METHODS
PRP-R/SRGF is a clinical-grade quality solution obtained from CaCl2 -activated platelets. Its activity was evaluated by measuring the proliferation, the phenotype, the differentiation potential and the immunosuppressive properties of MSCs derived from bone marrow (BM) and adipose tissue (AT).
RESULTS
PRP-R/SRGF was more active than FBS to expand BM- and AT-derived MSCs. PRP-R/SRGF treatment did not affect the expression of typical MSCs surface markers, neither MSCs differentiation potential nor their capability to inhibit activated T-cell proliferation.
CONCLUSIONS
The clinical-grade PRP-R/SRGF may be used in the clinical setting for the expansion of MSCs.
背景与目的
我们研究的目的是测试一种来自氯化钙激活血小板的富含血小板血浆释放物（PRP-R/SRGF），作为间充质基质细胞（MSCs）扩增的生长因子来源。通过低成本程序获得的PRP-R/SRGF，其特点是生长因子释放的变异性降低。
材料与方法
PRP-R/SRGF是一种从氯化钙激活血小板中获得的临床级质量溶液。通过测量源自骨髓（BM）和脂肪组织（AT）的MSCs的增殖、表型、分化潜能和免疫抑制特性来评估其活性。
结果
PRP-R/SRGF在扩增源自BM和AT的MSCs方面比胎牛血清（FBS）更具活性。PRP-R/SRGF处理不影响典型MSCs表面标志物的表达，也不影响MSCs的分化潜能及其抑制活化T细胞增殖的能力。
结论
临床级PRP-R/SRGF可用于临床环境中MSCs的扩增。
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