Herrera Pérez Zeneida, Weinfurter Stefanie, Gretz Norbert
Medical Research Center, Medical Faculty Mannheim, University of Heidelberg.
Medical Research Center, Medical Faculty Mannheim, University of Heidelberg;
J Vis Exp. 2016 Mar 26(109):e53767. doi: 10.3791/53767.
Glomerular filtration rate (GFR) is the gold standard to assess overall kidney function. However, traditional methods to evaluate GFR are cumbersome and time-consuming. In addition, serial blood or urine samples are required, with the associated stress for the experimental animals. A recent technique significantly reduces the investment in time and resources, minimizing the invasiveness and the animal stress, but being equally valid as the traditional approaches. The method measures transcutaneously renal function. Using an optical device and the exogenous renal marker fluorescein isothiocyanate (FITC)-sinistrin, this technique is capable of measuring the elimination kinetics of the marker through the skin. With neither blood nor urine samples nor the associated laboratory assays needed, the results of the transcutaneous measurement are almost instantaneously available. The method has been already validated in different species and successfully applied in several models of renal pathology. Moreover, due to its minimally invasive characteristics, it is suitable for sequential measurements within the same animal. Here is provided a detailed protocol to carry out the transcutaneous assessment of renal function in rodents.
肾小球滤过率（GFR）是评估整体肾功能的金标准。然而，传统的评估GFR的方法既繁琐又耗时。此外，还需要采集系列血液或尿液样本，这会给实验动物带来相应的应激。最近的一项技术显著减少了时间和资源投入，将侵入性和动物应激降至最低，但与传统方法同样有效。该方法通过经皮测量肾功能。利用光学设备和外源性肾脏标志物异硫氰酸荧光素（FITC）-左聚糖，这项技术能够通过皮肤测量标志物的消除动力学。由于既不需要采集血液或尿液样本，也不需要相关的实验室检测，经皮测量的结果几乎可以即时获得。该方法已在不同物种中得到验证，并成功应用于多种肾脏病理模型。此外，由于其微创特性，它适用于对同一动物进行连续测量。以下提供了一份详细的方案，用于对啮齿动物进行肾功能的经皮评估。
