Zhang Xiao-Xuan, Cong Wei, Liu Guo-Hua, Ni Xiao-Ting, Ma Jian-Gang, Zheng Wen-Bin, Zhao Quan, Zhu Xing-Quan
Acta Parasitol. 2016 Mar;61(2):382-8. doi: 10.1515/ap-2016-0050.
Enterocytozoon bieneusi is one of the most important zoonotic pathogen that can infect almost all animals, including humans. However, little information is available regarding prevalence and genotypes of E. bieneusi in sika deer. In the present study, the prevalence of E. bieneusi infection in sika deer in Jilin province, Northeastern China was examined using PCR amplification of the internal transcribed spacer (ITS) region of the ribosomal RNA (rRNA) gene. 23 (7.06%) of 326 samples were tested E. bieneusi-positive, and the risk factor significantly associated with E. bieneusi prevalence was the age of sika deer. Sequence analysis of the ITS rRNA gene suggested that 8 genotypes of E. bieneusi were found in this study, with five known genotypes, namely J (n = 11), BEB6 (n = 4), EbpC (n = 1), CHN-DC1 (n = 1), KIN-1 (n = 1) and three novel genotypes, namely JLD-1 (n = 2), JLD-2 (n = 2) and JLD-3 (n = 1). Phylogenetic analysis indicated that genotypes CHN-DC-1, KIN-1, EbpC, JLD-2 and JLD-3 fell into group 1, while other three genotypes (genotypes J, BEB6 and JLD-1) were clustered into group 2 (so-called bovine-specific groups). These findings indicated the presence of zoonotic E. bieneusi in Jilin province, Northeastern China. Effective strategies should be performed to control E. bieneusi infection in sika deer, other animals and humans.
微小隐孢子虫是最重要的人兽共患病原体之一，可感染几乎所有动物，包括人类。然而，关于梅花鹿体内微小隐孢子虫的流行情况和基因型的信息却很少。在本研究中，利用核糖体RNA（rRNA）基因内部转录间隔区（ITS）的PCR扩增技术，检测了中国东北吉林省梅花鹿体内微小隐孢子虫的感染率。326份样本中有23份（7.06%）检测出微小隐孢子虫呈阳性，与微小隐孢子虫流行率显著相关的危险因素是梅花鹿的年龄。ITS rRNA基因序列分析表明，本研究共发现8种微小隐孢子虫基因型，其中5种为已知基因型，即J型（n = 11）、BEB6型（n = 4）、EbpC型（n = 1）、CHN-DC1型（n = 1）、KIN-1型（n = 1），另外3种为新基因型，即JLD-1型（n = 2）、JLD-2型（n = 2）和JLD-3型（n = 1）。系统发育分析表明，CHN-DC-1型、KIN-1型、EbpC型、JLD-2型和JLD-3型属于第1组，而其他3种基因型（J型、BEB6型和JLD-1型）则聚为第2组（所谓的牛特异性组）。这些发现表明中国东北吉林省存在人兽共患的微小隐孢子虫。应采取有效的策略来控制梅花鹿、其他动物和人类体内微小隐孢子虫的感染。
