中国西南部马匹中微小隐孢子虫的分子特征及多位点基因型
Molecular characterization and multilocus genotypes of Enterocytozoon bieneusi among horses in southwestern China.
作者信息
Deng Lei, Li Wei, Zhong Zhijun, Gong Chao, Liu Xuehan, Huang Xiangming, Xiao Li, Zhao Ruoxuan, Wang Wuyou, Feng Fan, Zhang Yue, Hu Yanchun, Fu Hualin, He Min, Zhang Yue, Wu Kongju, Peng Guangneng
机构信息
The Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan Province, 611130, China.
Chengdu Giant Panda Breeding Research Base, Chengdu, Sichuan Province, 625001, China.
出版信息
Parasit Vectors. 2016 Oct 25;9(1):561. doi: 10.1186/s13071-016-1844-3.
BACKGROUND
Enterocytozoon bieneusi is one of the most prevalent causative species of diarrhea and enteric diseases in various hosts. E. bieneusi has been identified in humans, mammals, birds, rodents and reptiles in China, but few studies have reported E. bieneusi in horses. Therefore, the present study was conducted to assess the prevalence, molecular characteristics and zoonotic potential of E. bieneusi among horses in southwestern China.
FINDINGS
Three hundred and thirty-three fecal specimens were collected from horses on five farms in the Sichuan and Yunnan provinces of southwestern China. The prevalence of E. bieneusi was 22.5 % (75/333), as determined by nested polymerase chain reaction and sequencing analysis of the internal transcribed spacer region of the ribosomal RNA gene of E. bieneusi. Altogether, 10 genotypes were identified among the 75 E. bieneusi-positive samples: four of these genotypes were known (horse1, horse2, SC02 and D) and six were novel (SCH1-4 and YNH1-2). Multilocus sequence typing using three microsatellites (MS1, MS3 and MS7) and one minisatellite (MS4) revealed three, two, three and three genotypes at these four loci, respectively. In phylogenetic analysis, all the genotypes of E. bieneusi obtained in this study were clustered into three distinct groups: D, SC02 and SCH1-3 were clustered into group 1 (zoonotic potential); SCH4 was clustered into group 2 (cattle-hosted); whereas horse2, YNH1 and YNH2 were clustered into group 6 (unclear zoonotic potential).
CONCLUSIONS
This is the first report of E. bieneusi among horses in southwestern China. This is also the first multilocus genotyping analysis using microsatellite and minisatellite markers of E. bieneusi in horses. The presence of genotype D, which was previously identified in humans, and genotypes SC02 and SCH1-3, which belong to potential zoonotic group 1, these results indicate that horses are a potential source of human E. bieneusi infections in China.
背景
微小隐孢子虫是各种宿主中引起腹泻和肠道疾病的最常见病原体之一。在中国，微小隐孢子虫已在人类、哺乳动物、鸟类、啮齿动物和爬行动物中被鉴定出来，但关于马体内微小隐孢子虫的研究报道较少。因此，本研究旨在评估中国西南部马匹中微小隐孢子虫的流行情况、分子特征和人畜共患病潜力。
研究结果
从中国西南部四川省和云南省的五个农场的马匹中收集了333份粪便样本。通过巢式聚合酶链反应和对微小隐孢子虫核糖体RNA基因内部转录间隔区的测序分析，确定微小隐孢子虫的感染率为22.5%（75/333）。在75份微小隐孢子虫阳性样本中共鉴定出10种基因型：其中4种基因型是已知的（horse1、horse2、SC02和D），6种是新的（SCH1 - 4和YNH1 - 2）。使用三个微卫星（MS1、MS3和MS7）和一个小卫星（MS4）进行多位点序列分型，分别在这四个位点揭示了3种、2种、3种和3种基因型。在系统发育分析中，本研究获得的所有微小隐孢子虫基因型被聚类为三个不同的组：D、SC02和SCH1 - 3聚类到第1组（有人畜共患病潜力）；SCH4聚类到第2组（以牛为宿主）；而horse2、YNH1和YNH2聚类到第6组（人畜共患病潜力不明）。
结论
这是中国西南部马匹中微小隐孢子虫的首次报道。这也是首次在马匹中使用微卫星和小卫星标记对微小隐孢子虫进行多位点基因分型分析。先前在人类中鉴定出的基因型D以及属于潜在人畜共患病第1组的基因型SC02和SCH1 - 3的存在，这些结果表明马匹是中国人类微小隐孢子虫感染的潜在来源。
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