Wang Fang, Guo Weil, Ma Peng-kun, Pan Liang, Zhang Jun
Huan Jing Ke Xue. 2016 Jan 15;37(1):309-16.
A greenhouse pot experiment was conducted to investigate the effects of arbuscular mycorrhizal (AM) fungi Glomus aggregatum (GA) and Funneliformis mosseae (FM) on AM colonization rate, biomass, nutrient uptake, C: N: P stoichiometric and Ce uptake and transport by maize (Zea mays L.) grown in soils with different levels of Ce-contaminated (100, 500 and 1000 mg x kg(-1)). The aim was to provide basic data and technical support for the treatment of soils contaminated by rare earth elements. The results indicated that symbiotic associations were successfully established between the two isolates and maize, and the average AM colonization rate ranged from 7. 12% to 74.47%. The increasing concentration of Ce in soils significantly decreased the mycorrhizal colonization rate, biomass, nutrition contents and transport rate of Ce from root to shoot of maize, and significantly increased C: P and N: P ratios and Ce contents in shoot and root of maize. Both AM fungi inoculations promoted the growth of maize, but the promoting role of FM was more significant than that of GA in severe Ce-contaminated soils. There were no significant differences in the growth of maize between two AM fungi in mild and moderate Ce-contaminated soils. Inoculation with AM fungi significantly improved nutritional status of maize by increasing nutrient uptake and decreasing C: N: P ratios. GA was more efficient than FM in enhancing nutrient uptake in mild and moderate Ce-contaminated soils, while FM was more efficient in severe Ce-contaminated soils. Moreover, inoculation with AM fungi significantly increased Ce contents of shoot and root in mild Ce-contaminated soils, but had no significant effect on Ce contents of maize in moderate and severe Ce-contaminated soils, and promoted the transport of Ce from root to shoot. The experiment demonstrates that AM fungi can alleviate toxic effects of Ce on plants and have a potential role in the phytoremediation of soils contaminated by rare earth elements.
进行了一项温室盆栽试验，以研究丛枝菌根（AM）真菌聚球囊霉（GA）和摩西管柄囊霉（FM）对生长在不同铈污染水平（100、500和1000 mg·kg⁻¹）土壤中的玉米（Zea mays L.）的AM定殖率、生物量、养分吸收、碳氮磷化学计量比以及铈吸收和转运的影响。目的是为稀土元素污染土壤的治理提供基础数据和技术支持。结果表明，两种分离菌株与玉米成功建立了共生关系，平均AM定殖率在7.12%至74.47%之间。土壤中铈浓度的增加显著降低了玉米的菌根定殖率、生物量、营养含量以及铈从根到地上部的转运速率，并显著提高了玉米地上部和根部的碳磷比和氮磷比以及铈含量。两种AM真菌接种均促进了玉米生长，但在重度铈污染土壤中，FM的促进作用比GA更显著。在轻度和中度铈污染土壤中，两种AM真菌对玉米生长的促进作用无显著差异。接种AM真菌通过增加养分吸收和降低碳氮磷比显著改善了玉米的营养状况。在轻度和中度铈污染土壤中，GA在增强养分吸收方面比FM更有效，而在重度铈污染土壤中FM更有效。此外，接种AM真菌显著增加了轻度铈污染土壤中玉米地上部和根部的铈含量，但对中度和重度铈污染土壤中玉米的铈含量无显著影响，并促进了铈从根到地上部的转运。该实验表明，AM真菌可以减轻铈对植物的毒害作用，在稀土元素污染土壤的植物修复中具有潜在作用。
