Kheirbakhsh Raheleh, Chinisaz Maryam, Amanpour Saeid, Amini Shahab, Khodayari Saeed, Khodayari Hamid, Dilmaghanian Aydin, Haddadi Mahnaz, Ebrahim-Habibi Azadeh
a Endocrinology and Metabolism Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences , Tehran , Iran.
b Biosensor Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences , Tehran , Iran.
Drug Chem Toxicol. 2017 Jan;40(1):1-6. doi: 10.3109/01480545.2016.1163575. Epub 2016 Apr 14.
Protein-derived amyloid structures are associated with a wide variety of pathologies, including neurodegenerative diseases and local amyloidoses. Reports exist on the ability of insulin to form local amyloidoses under specific conditions. In vitro-generated fibrils of insulin have been previously shown to produce amyloid-containing masses upon repetitive subcutaneous injection in mouse. The present study aimed at investigating the effect of insulin fibrils injection in rats, as well as the potential of turmeric in attenuating this process. It was found that subcutaneous amyloid-containing masses could form in rats at a faster rate compared with mice. Upon addition of turmeric to the fibrils, previous to injection, formed masses had a significantly reduced size, as well as less ordered cellular structure. In conclusion, the results of this study show the potential of turmeric in attenuation of local amyloidosis. Furthermore, we suggest that this model could be of use in screening antiamyloid compounds.
蛋白质衍生的淀粉样结构与多种病理状况相关，包括神经退行性疾病和局部淀粉样变性。有关于胰岛素在特定条件下形成局部淀粉样变性的能力的报道。先前已表明，体外生成的胰岛素原纤维在小鼠体内重复皮下注射后会产生含淀粉样物质的团块。本研究旨在调查胰岛素原纤维注射对大鼠的影响，以及姜黄在减轻这一过程中的潜力。结果发现，与小鼠相比，大鼠皮下含淀粉样物质的团块形成速度更快。在注射前将姜黄添加到原纤维中后，形成的团块大小显著减小，细胞结构的有序性也降低。总之，本研究结果显示了姜黄在减轻局部淀粉样变性方面的潜力。此外，我们认为该模型可用于筛选抗淀粉样化合物。
