Sandgren Per, Berlin Gösta, Tynngård Nahreen
Department of Clinical Immunology and Transfusion Medicine, Karolinska University Hospital and Karolinska Institutet, Stockholm, Sweden.
Department of Clinical Immunology and Transfusion Medicine, Linköping University, Linköping, Sweden.
Transfusion. 2016 Jun;56(6):1377-83. doi: 10.1111/trf.13601. Epub 2016 Apr 15.
Pathogen reduction technologies use photoactive substances in combination with ultraviolet (UV) light to inactivate pathogens. A new method uses only UVC light for pathogen reduction. This study assesses the effects of UVC light treatment on cytokine release in platelet (PLT) concentrates (PCs).
A PC with 35% plasma and 65% PLT additive solution (SSP+) was prepared from five buffy coats. Three such PCs were pooled and divided into 3 units. One unit was used as a nonirradiated control, the second was a gamma-irradiated control, and the third unit was treated with UVC light technology. Ten units of each type were investigated. Cytokine release was analyzed on Days 1, 5, and 7 of storage. Correlation between cytokines, PLT surface markers, and hemostatic properties was investigated.
Swirling was well preserved and pH was above the reference limit of 6.4 during storage of PLTs in all groups. Cytokine levels increased during storage in all groups but to a larger degree in PCs treated with UVC light. Only weak correlation was found between cytokines and PLT surface markers (r < 0.5). However, several cytokines showed strong correlation (r > 0.6) with the PLTs' ability to promote clot retraction.
UVC treatment resulted in increased release from PLT alpha granules as evident by a higher cytokine release compared to nonirradiated and gamma-irradiated PCs. The clinical relevance of these findings needs to be further evaluated.
病原体灭活技术利用光活性物质与紫外线（UV）结合来灭活病原体。一种新方法仅使用UVC光来减少病原体。本研究评估了UVC光处理对血小板（PLT）浓缩物（PC）中细胞因子释放的影响。
从五个白膜层制备含35%血浆和65%PLT添加剂溶液（SSP+）的PC。将三个这样的PC合并并分成3个单位。一个单位用作未辐照对照，第二个用作γ辐照对照，第三个单位用UVC光技术处理。每种类型研究10个单位。在储存的第1、5和7天分析细胞因子释放。研究了细胞因子、PLT表面标志物和止血特性之间的相关性。
在所有组中，血小板储存期间漩涡现象保存良好，pH高于参考限值6.4。所有组中细胞因子水平在储存期间均升高，但在用UVC光处理的PC中升高程度更大。细胞因子与PLT表面标志物之间仅发现弱相关性（r < 0.5）。然而，几种细胞因子与血小板促进血块回缩的能力显示出强相关性（r > 0.6）。
与未辐照和γ辐照的PC相比，UVC处理导致PLTα颗粒释放增加，细胞因子释放更高表明了这一点。这些发现的临床相关性需要进一步评估。
