Tynngård Nahreen, Trinks Marie, Berlin Gösta
Department of Clinical Immunology and Transfusion Medicine, Linköping University, Linköping, Sweden.
Department of Clinical and Experimental Medicine, Linköping University, Linköping, Sweden.
Transfusion. 2015 Jun;55(6):1169-77. doi: 10.1111/trf.12963. Epub 2014 Dec 18.
During storage of platelet concentrates (PCs) replication of contaminating pathogens might occur, which can be prevented by various pathogen inactivation (PI) methods using photoactive substances in combination with ultraviolet (UV) light. A new method uses only UVC light for PI without photoactive substances. This study evaluates the in vitro function, including hemostatic properties (clot formation and elasticity), of platelets (PLTs) treated with UVC light.
A PC with 35% plasma and 65% PLT additive solution (SSP+) was prepared from five buffy coats. Three PCs were pooled and divided into 3 units. One unit was used as a nonirradiated control, the second was a gamma-irradiated control, and the third unit was treated with UVC light. In vitro variables including analysis of coagulation by free oscillation rheometry were analyzed on Days 1, 5, and 7 of storage. Ten units in each group were investigated.
Swirling was well preserved, and the pH level was higher than the reference limit (6.4) during storage of PLTs in all groups. Glycolysis and PLT activation were higher for UVC-treated PLTs but the clot-forming capacity was unaffected. However, immediately after UVC treatment, the clot elastic properties were slightly affected. Hypotonic shock response decreased immediately after UVC treatment but recovered partly during the storage period.
UVC treatment affected the in vitro properties, but PLT quality and storage stability were well preserved for up to 7 days, and the in vitro hemostatic capacity of UVC-treated PLTs was only minimally altered. The clinical relevance of these changes needs to be evaluated in controlled trials.
在血小板浓缩物（PCs）储存期间，污染病原体可能会复制，这可以通过使用光活性物质与紫外线（UV）光结合的各种病原体灭活（PI）方法来预防。一种新方法仅使用UVC光进行病原体灭活，无需光活性物质。本研究评估了经UVC光处理的血小板（PLTs）的体外功能，包括止血特性（凝血形成和弹性）。
从五个 Buffy 层制备了含 35%血浆和 65%血小板添加剂溶液（SSP+）的 PC。将三个 PC 合并并分成 3 个单位。一个单位用作未辐照对照，第二个用作γ辐照对照，第三个单位用 UVC 光处理。在储存的第 1、5 和 7 天分析体外变量，包括通过自由振荡流变学分析凝血。每组研究 10 个单位。
在所有组中，血小板储存期间漩涡现象保存良好，pH 值高于参考限值（6.4）。UVC 处理的血小板糖酵解和血小板活化较高，但凝血形成能力未受影响。然而，UVC 处理后立即，凝血弹性特性略有影响。UVC 处理后低渗休克反应立即下降，但在储存期部分恢复。
UVC 处理影响体外特性，但血小板质量和储存稳定性在长达 7 天内保存良好，UVC 处理的血小板体外止血能力仅略有改变。这些变化的临床相关性需要在对照试验中进行评估。
