Wesener Thomas, Voigtländer Karin, Decker Peter, Oeyen Jan Philip, Spelda Jörg
Zoologisches Forschungsmuseum Alexander Koenig, Leibniz Institute for Animal Biodiversity, Center for Taxonomy and Evolutionary Research (Section Myriapoda), Adenauerallee 160, 53113 Bonn, Germany.
Senckenberg Museum of Natural History Görlitz, Am Museum 1, 02826 Görlitz, Germany.
Zookeys. 2016 Feb 16(564):21-46. doi: 10.3897/zookeys.564.7535. eCollection 2016.
In order to evaluate the diversity of Central European Myriapoda species in the course of the German Barcode of Life project, 61 cytochrome c oxidase I sequences of the genus Cryptops Leach, 1815, a centipede genus of the order Scolopendromorpha, were successfully sequenced and analyzed. One sequence of Scolopendra cingulata Latreille, 1829 and one of Theatops erythrocephalus Koch, 1847 were utilized as outgroups. Instead of the expected three species (Cryptops parisi Brolemann, 1920; Cryptops anomalans Newport, 1844; Cryptops hortensis (Donovan, 1810)), analyzed samples included eight to ten species. Of the eight clearly distinguishable morphospecies of Cryptops, five (Cryptops parisi; Cryptops croaticus Verhoeff, 1931; Cryptops anomalans; Cryptops umbricus Verhoeff, 1931; Cryptops hortensis) could be tentatively determined to species level, while a further three remain undetermined (one each from Germany, Austria and Croatia, and Slovenia). Cryptops croaticus is recorded for the first time from Austria. A single specimen (previously suspected as being Cryptops anomalans), was redetermined as Cryptops umbricus Verhoeff, 1931, a first record for Germany. All analyzed Cryptops species are monophyletic and show large genetic distances from one another (p-distances of 13.7-22.2%). Clear barcoding gaps are present in lineages represented by >10 specimens, highlighting the usefulness of the barcoding method for evaluating species diversity in centipedes. German specimens formally assigned to Cryptops parisi are divided into three clades differing by 8.4-11.3% from one another; their intra-lineage genetic distance is much lower at 0-1.1%. The three clades are geographically separate, indicating that they might represent distinct species. Aside from Cryptops parisi, intraspecific distances of Cryptops spp. in Central Europe are low (<3.3%).
为了在德国生命条形码项目过程中评估中欧多足纲物种的多样性，成功测序并分析了隐蜈蚣属（Cryptops Leach，1815）的61条细胞色素c氧化酶I序列，隐蜈蚣属是蜈蚣目蜈蚣亚目的一个属。选取了1829年拉特雷尔描述的少棘蜈蚣（Scolopendra cingulata）的一条序列以及1847年科赫描述的红头蜈蚣（Theatops erythrocephalus）的一条序列作为外类群。分析的样本中包含八到十个物种，而非预期的三个物种（巴黎隐蜈蚣（Cryptops parisi Brolemann，1920）、异常隐蜈蚣（Cryptops anomalans Newport，1844）、园圃隐蜈蚣（Cryptops hortensis (Donovan, 1810)））。在隐蜈蚣属的八个明显可区分的形态物种中，有五个（巴黎隐蜈蚣、克罗地亚隐蜈蚣（Cryptops croaticus Verhoeff，1931）、异常隐蜈蚣、荫蔽隐蜈蚣（Cryptops umbricus Verhoeff，1931）、园圃隐蜈蚣）可初步确定到物种水平，另外三个仍未确定（分别来自德国、奥地利、克罗地亚和斯洛文尼亚各一个）。克罗地亚隐蜈蚣首次在奥地利被记录。一个曾被怀疑为异常隐蜈蚣的单一标本，被重新确定为1931年韦尔霍夫描述的荫蔽隐蜈蚣，这是在德国的首次记录。所有分析的隐蜈蚣物种都是单系的，并且彼此之间显示出较大的遗传距离（p距离为13.7 - 22.2%）。在由超过10个标本代表的谱系中存在明显的条形码间隙，突出了条形码方法在评估蜈蚣物种多样性方面的有用性。正式归为巴黎隐蜈蚣的德国标本被分为三个分支，彼此之间相差8.4 - 11.3%；它们谱系内的遗传距离要低得多，为0 - 1.1%。这三个分支在地理上是分开的，表明它们可能代表不同的物种。除巴黎隐蜈蚣外，中欧隐蜈蚣属物种的种内距离较低（<3.3%）。
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