Rarey K E, Patterson K
Department of Anatomy, College of Medicine, University of Florida, Gainesville 32610.
Hear Res. 1989 Apr;38(3):277-87. doi: 10.1016/0378-5955(89)90071-3.
Select epithelial regions of the bovine inner ear were established and maintained in cell culture. Marginal cells from the stria vascularis and dark cells from the posterior wall of the utricle were isolated, dissociated and placed in culture medium. Within 24 h, cellular islands of hexagonal-shaped, epithelial-like cells from both the stria vascularis and posterior utricular wall were readily identifiable by inverted light microscopy. Ultrastructural examination of both the cultured stria marginal cells and utricular dark cells revealed that both cell types had numerous microvilli on their apical surfaces and interdigitating infoldings of their basolateral surfaces. Apical tight junctional complexes were present between apposing cells. These findings demonstrate that inner ear bovine epithelial cells can be successfully isolated and maintained in culture, and that such cells retain certain of their in vivo morphological characteristics.
建立并维持了牛内耳的特定上皮区域的细胞培养。分离了血管纹的边缘细胞和椭圆囊后壁的暗细胞，将其解离并置于培养基中。在24小时内，通过倒置光学显微镜很容易识别出血管纹和椭圆囊后壁的六边形上皮样细胞组成的细胞岛。对培养的血管纹边缘细胞和椭圆囊暗细胞的超微结构检查显示，这两种细胞类型在其顶端表面都有大量微绒毛，在其基底外侧表面有相互交错的褶皱。相邻细胞之间存在顶端紧密连接复合体。这些发现表明，牛内耳上皮细胞可以成功地分离并在培养中维持，并且这些细胞保留了它们在体内的某些形态特征。
