Yang Guanghua, Zong Huajie
Department of General Surgery, Huashan Hospital Affiliated to Fudan University, Shanghai 200040, P.R. China.
Mol Med Rep. 2016 Jun;13(6):4786-90. doi: 10.3892/mmr.2016.5110. Epub 2016 Apr 12.
Intrahepatic cholangiocarcinoma (iCCA) is an aggressive malignancy in the liver, which is associated with a poor prognosis. However, the molecular pathogenesis of iCCA remains unclear. RNA-Seq for tumor and para-tumor sample pairs enables the characterization of changes in the gene expression profiles of patients with iCCA. The present study analyzed RNA‑Seq data of seven iCCA para‑tumor and tumor sample pairs. Differential gene expression analysis demonstrated significant upregulation of PDZK1IP1, EEF1A2 and RPL41 (ENSG00000279483) genes in the iCCA samples when compared with the matched para‑tumor samples. Furthermore, genes associated with the immune system, metabolism and metabolic energy were significantly downregulated in the iCCA tumor tissues, indicating that this is involved in the pathogenesis of iCCA. The present study aimed to elucidate the gene expression patterns associated with the tumorigenesis of iCCA by comparing tumor and normal tissues, in order to isolate novel diagnostic factors for iCCA.
肝内胆管癌（iCCA）是一种侵袭性肝脏恶性肿瘤，预后较差。然而，iCCA的分子发病机制仍不清楚。对肿瘤和癌旁样本对进行RNA测序能够表征iCCA患者基因表达谱的变化。本研究分析了7对iCCA癌旁和肿瘤样本的RNA测序数据。差异基因表达分析表明，与匹配的癌旁样本相比，iCCA样本中PDZK1IP1、EEF1A2和RPL41（ENSG00000279483）基因显著上调。此外，与免疫系统、代谢和代谢能量相关的基因在iCCA肿瘤组织中显著下调，表明这与iCCA的发病机制有关。本研究旨在通过比较肿瘤组织和正常组织来阐明与iCCA肿瘤发生相关的基因表达模式，以便分离出iCCA的新型诊断因子。
