Fast D M, Reddy V V, Ashley D L, Holler J S
Centers for Disease Control, Division of Environmental Health Laboratory Sciences, Atlanta, GA 30333.
J Assoc Off Anal Chem. 1989 Mar-Apr;72(2):378-83.
Pure analytical standards are key components in the successful development of accurate analytical methods. When such compounds are unavailable commercially, a laboratory synthesis activity can provide such materials for use in method development. An example of such an approach is the synthesis of ethyl ethers of chlorinated phenols and ethyl esters of several phenoxy herbicides. Individual synthesis steps and the characterization of such materials are described. The use of such analytical standards in method development is vital for defining method characteristics. In addition, such materials are helpful in diagnosing chromatographic and mass spectral problems in the course of this work. Key mass spectral information including parent and daughter scans obtained from these analytical standards by using tandem mass spectrometry provide the basis for a new analytical method with increased sensitivity and specificity.
纯分析标准品是成功开发准确分析方法的关键组成部分。当此类化合物无法从商业渠道获得时，实验室合成活动可提供此类材料用于方法开发。这种方法的一个例子是合成氯酚的乙醚和几种苯氧基除草剂的乙酯。描述了各个合成步骤以及此类材料的表征。在方法开发中使用此类分析标准品对于定义方法特性至关重要。此外，此类材料有助于在这项工作过程中诊断色谱和质谱问题。通过串联质谱从这些分析标准品获得的关键质谱信息，包括母离子和子离子扫描，为一种具有更高灵敏度和特异性的新分析方法提供了基础。
