天蓝色链霉菌中的谷氨酸合成。
Glutamate synthesis in Streptomyces coelicolor.
作者信息
Fisher S H
机构信息
Department of Microbiology, Boston University School of Medicine, Massachusetts 02118.
出版信息
J Bacteriol. 1989 May;171(5):2372-7. doi: 10.1128/jb.171.5.2372-2377.1989.
Abstract
Both glutamate synthase (GOGAT) and glutamate dehydrogenase (GDH) are involved in glutamate synthesis in Streptomyces coelicolor. The highest levels of GDH were seen in extracts of cells grown with high levels of ammonium as the nitrogen source. GOGAT activity was reduced two- to threefold in extracts of cells grown with good sources of glutamate. S. coelicolor mutants deficient in GOGAT (Glt-) required glutamate for growth with L-alanine, asparagine, arginine, or histidine as the nitrogen source but grew like wild-type cells when ammonium, glutamine, or aspartate was the nitrogen source. The glt mutations were tightly linked to hisA1. Mutants deficient in both GOGAT and GDH (Gdh-) required glutamate for growth in all media. The gdh-5 mutation was mapped to the left region of the S. coelicolor chromosomal map, between proA1 and uraA1.
摘要
谷氨酸合酶（GOGAT）和谷氨酸脱氢酶（GDH）都参与天蓝色链霉菌中的谷氨酸合成。在以高浓度铵作为氮源生长的细胞提取物中，GDH水平最高。在用优质谷氨酸源培养的细胞提取物中，GOGAT活性降低了两到三倍。缺乏GOGAT（Glt-）的天蓝色链霉菌突变体在以L-丙氨酸、天冬酰胺、精氨酸或组氨酸作为氮源生长时需要谷氨酸，但当铵、谷氨酰胺或天冬氨酸作为氮源时，其生长情况与野生型细胞相似。glt突变与hisA1紧密连锁。同时缺乏GOGAT和GDH（Gdh-）的突变体在所有培养基中生长都需要谷氨酸。gdh-5突变被定位到天蓝色链霉菌染色体图谱的左侧区域，位于proA1和uraA1之间。
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