Raport C J, Dere B, Hurley J B
Department of Biochemistry, University of Washington, Seattle 98195.
J Biol Chem. 1989 May 5;264(13):7122-8.
A genomic clone spanning the mouse rod transducin alpha subunit (Tr alpha) gene has been isolated by screening a mouse genomic library with a bovine Tr alpha cDNA clone. The coding region of the mouse Tr alpha gene reveals an 88.7% nucleotide identify and 99.7% amino acid identity with bovine Tr alpha. The mouse Tr alpha gene is composed of 8 exons and 7 introns within its coding region. These introns are in the same locations as introns in human Gi alpha genes, that encode G proteins closely related to transducin. Primer extension, RNA sequencing, and S1 nuclease protection analyses indicate that the mouse Tr alpha gene transcription start site is 84 bases upstream of the initiation codon. Northern blot analysis shows that the mouse Tr alpha is expressed in the retina, but not in brain, kidney, liver, or heart.
通过用牛转导蛋白α亚基（Trα）cDNA克隆筛选小鼠基因组文库，分离出了一个跨越小鼠视杆转导蛋白α亚基（Trα）基因的基因组克隆。小鼠Trα基因的编码区与牛Trα的核苷酸同一性为88.7%，氨基酸同一性为99.7%。小鼠Trα基因在其编码区内由8个外显子和7个内含子组成。这些内含子与人类Giα基因中的内含子位于相同位置，人类Giα基因编码与转导蛋白密切相关的G蛋白。引物延伸、RNA测序和S1核酸酶保护分析表明，小鼠Trα基因转录起始位点在起始密码子上游84个碱基处。Northern印迹分析显示，小鼠Trα在视网膜中表达，但在脑、肾、肝或心脏中不表达。
