al-Timimi I, Gaillard J L, Amri H, Silberzahn P
Laboratoire de Biochimie, Unité Associée au Centre National de la Recherche Scientifique, Université, Caen, France.
J Biol Chem. 1989 May 5;264(13):7161-8.
Whereas mare corpus luteum does not produce androgens or estrogens in vivo, the incubation of mare corpus luteum microsomes with progesterone and NADPH resulted in 17 alpha-hydroxyprogesterone and estrogen production with a small yield of androstenedione. In the presence of an aromatase inhibitor (4-hydroxyandrostenedione), 17 alpha-hydroxyprogesterone and androstenedione were accumulated. Aromatization of testosterone and androstenedione occurred via stereospecific loss of the 1 beta, 2 beta hydrogen atoms and was inhibited by MgCl2, KCl, and EDTA. The Km of estrogen synthetase from equine corpus luteum for testosterone was 18.5 +/- 2.7 nM and for androstenedione was 11.5 +/- 1.5 nM. 19-Norandrogens were aromatized with a slightly higher efficiency than were androgens, but the affinity of the aromatase was lower for 19-norandrogens than for androgens. Our results suggest that aromatases from equine testis and corpus luteum are closely related enzymes. On the other hand, the question arises as to the relationship among the cell origin, the synthetizing abilities, and in vivo production of the corpus luteum in different mammalian species.
虽然母马黄体在体内不产生雄激素或雌激素，但将母马黄体微粒体与孕酮和烟酰胺腺嘌呤二核苷酸磷酸（NADPH）一起孵育会产生17α-羟孕酮和雌激素，同时生成少量雄烯二酮。在存在芳香化酶抑制剂（4-羟基雄烯二酮）的情况下，17α-羟孕酮和雄烯二酮会积累。睾酮和雄烯二酮的芳香化作用通过1β、2β氢原子的立体特异性丢失而发生，并受到氯化镁、氯化钾和乙二胺四乙酸（EDTA）的抑制。马黄体雌激素合成酶对睾酮的米氏常数（Km）为18.5±2.7纳摩尔，对雄烯二酮的Km为11.5±1.5纳摩尔。19-去甲雄激素的芳香化效率略高于雄激素，但芳香化酶对19-去甲雄激素的亲和力低于对雄激素的亲和力。我们的结果表明，马睾丸和黄体的芳香化酶是密切相关的酶。另一方面，不同哺乳动物物种黄体的细胞起源、合成能力和体内产生之间的关系问题也随之出现。
