Ito Y, Sisido M, Imanishi Y
Department of Polymer Chemistry, Faculty of Engineering, Kyoto University, Japan.
J Biomed Mater Res. 1989 Feb;23(2):191-206. doi: 10.1002/jbm.820230205.
Interactions of platelet with novel polyetherurethaneurea and its heparinized derivative were investigated. Platelet adhesion onto the material and release of serotonin or adenosine phosphate from platelet-rich plasma (PRP) were suppressed by an introduction of amino groups to polyetherurethaneurea, by quaternization of the polymer, and further by heparinization of the polymer. When the material was precoated with one of major plasma proteins and the protein-coated materials were taken to contact with washed platelet suspension (WP), the dependence of platelet adhesion and activation on the properties of polymers was different from that observed for PRP interaction. Platelet adhesion and activation were promoted according to the nature of coating proteins in the order albumin less than gamma-globulin less than fibrinogen and with increasing degree of denaturation of coating proteins. When the polymer materials were coated with proteins by immersing in aqueous solution containing two kinds of plasma proteins, adhesion behaviors of platelet were similar to those observed for PRP-uncoated material interaction. These experimental facts indicate that the selectivity of platelet for protein-coated material cannot be assessed by the interaction of WP with materials coated with a single kind of protein. It was concluded that material surface to which albumin is selectively adsorbed without denaturation does not stimulate adhering platelets for release reactions.
研究了血小板与新型聚醚聚氨酯脲及其肝素化衍生物的相互作用。通过向聚醚聚氨酯脲引入氨基、使聚合物季铵化以及进一步使聚合物肝素化，可抑制血小板在材料上的黏附以及富含血小板血浆（PRP）中5-羟色胺或磷酸腺苷的释放。当材料预先用一种主要血浆蛋白包被，且将蛋白包被的材料与洗涤后的血小板悬液（WP）接触时，血小板黏附与活化对聚合物性质的依赖性与PRP相互作用时观察到的情况不同。血小板的黏附与活化根据包被蛋白的性质按白蛋白＜γ-球蛋白＜纤维蛋白原的顺序以及随着包被蛋白变性程度的增加而增强。当通过将聚合物材料浸入含有两种血浆蛋白的水溶液中使其被蛋白包被时，血小板的黏附行为类似于未包被PRP的材料相互作用时观察到的情况。这些实验事实表明，血小板对蛋白包被材料的选择性不能通过WP与单一种类蛋白包被材料的相互作用来评估。得出的结论是，未变性情况下被白蛋白选择性吸附的材料表面不会刺激黏附的血小板发生释放反应。
