Souto Giovanna Ribeiro, Nunes Laiz Fernandes Mendes, Tanure Barbara Brandão, Gomez Ricardo Santiago, Mesquita Ricardo Alves
Department of Oral Surgery and Pathology, School of Dentistry, Universidade Federal de Minas Gerais, Belo Horizonte, Brasil; Hospital Público Regional de Betim; Faculdade de Odontologia Sete Lagoas (FACSETE), Minas Gerais, Brazil.
Department of Oral Surgery and Pathology, School of Dentistry, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
Oral Surg Oral Med Oral Pathol Oral Radiol. 2016 Jun;121(6):651-6. doi: 10.1016/j.oooo.2016.02.009. Epub 2016 Feb 23.
To evaluate and compare the number of CD1a+ dendritic cells (DCs) in oral lichen planus (OLP), amalgam lichenoid reaction (ALR), and normal oral mucosa (NOM).
Fourteen cases of OLP and ALR, diagnosed both clinically and histologically, were obtained from an archive of an oral pathology service. Seven specimens of NOM were collected and served as controls. Immunohistochemistry was used to identify CD1a+ DCs. Densities were calculated using specimens of epithelium and lamina propria. For OLP and ALR, specimens of lamina propria included cells from inflammatory infiltrate (region A), and from below inflammatory infiltrate (region B). Comparisons and statistical analyses were performed among OLP, ALR, and NOM.
A higher density of CD1a+ DCs was observed in OLP compared to ALR in region A (P < .05) and regions A+B (P < .05). A higher density of CD1a+ DCs was also observed in OLP compared with NOM in the regions A+B (P < .05). Statistically significant differences were not observed for analyses involving epithelial tissue.
The observation that there is a lower quantity of DCs in ALR specimens compared with specimens from individuals with OLP suggests that the role for DCs in the 2 diseases may be different. The difference in cell density could be used in the differential diagnosis of these 2 diseases, but the overlapping intervals in the statistical analysis may limit the application of this approach in surgical practice.
评估并比较口腔扁平苔藓（OLP）、汞合金苔藓样反应（ALR）和正常口腔黏膜（NOM）中CD1a⁺树突状细胞（DCs）的数量。
从口腔病理学服务档案中获取14例经临床和组织学诊断的OLP和ALR病例。收集7份NOM标本作为对照。采用免疫组织化学法鉴定CD1a⁺DCs。使用上皮和固有层标本计算密度。对于OLP和ALR，固有层标本包括来自炎症浸润区（A区）和炎症浸润区下方（B区）的细胞。对OLP、ALR和NOM进行比较和统计分析。
在A区（P <.05）和A + B区（P <.05），OLP中CD1a⁺DCs的密度高于ALR。在A + B区，OLP中CD1a⁺DCs的密度也高于NOM（P <.05）。涉及上皮组织的分析未观察到统计学上的显著差异。
与OLP患者的标本相比，ALR标本中DCs数量较少，这一观察结果表明DCs在这两种疾病中的作用可能不同。细胞密度的差异可用于这两种疾病的鉴别诊断，但统计分析中的重叠区间可能会限制这种方法在外科实践中的应用。
