Gustafson Jenny, Eklund Christina, Wallström Mats, Zellin Göran, Magnusson Bengt, Hasséus Bengt
Section of Oral Immunology/Clinic for Oral Medicine, The Sahlgrenska Academy at Göteborg University, Göteborg, Sweden.
Acta Odontol Scand. 2007 Jun;65(3):156-61. doi: 10.1080/00016350601137251.
Dendritic Langerhans cells (LCs) have been attributed a role in the pathogenesis of lichen planus as autoantigen-presenting cells initiating expansion of autoreactive T cells. Langerin and CD83, which are cell molecules expressed on LCs, are associated with antigen presentation. The present study examined expression of Langerin and CD83 molecules on LCs in patients with oral lichen planus (OLP).
Biopsies were obtained from seven patients with OLP. Oral mucosa from seven healthy subjects served as controls. Monoclonal antibodies (mAbs) were used in standard immunohistochemical procedures to visualize CD1a-, Langerin-, and CD83-molecule-expressing cells.
CD1a+ and Langerin+ cells were found in significantly higher frequencies in OLP epithelium compared with healthy oral epithelium (p<0.01 and p<0.05, respectively); however, the frequency of CD83+ cells did not differ (p>0.05). The connective tissue in OLP lesions showed significantly higher frequencies of CD1a+, Langerin+, and CD83+ cells compared with healthy connective tissue (p<0.01, p<0.01, and p<0.05). CD1a+ and Langerin+ cells in OLP and healthy epithelium had a dendritic morphology.
The study shows increased numbers of CD1a- and Langerin-expressing LCs in OLP compared with healthy controls. In the connective tissue, CD83+ cells with dendritic morphology were localized to regions of lymphocyte clusters. The presence of CD83+ dendritic cells in areas of lymphocyte clusters in the connective tissue of OLP lesions indicates the possibility of ongoing autoantigen presentation.
树突状朗格汉斯细胞（LCs）在扁平苔藓的发病机制中被认为具有一定作用，作为自身抗原呈递细胞启动自身反应性T细胞的扩增。Langerin和CD83是在LCs上表达的细胞分子，与抗原呈递相关。本研究检测了口腔扁平苔藓（OLP）患者LCs上Langerin和CD83分子的表达情况。
从7例OLP患者身上获取活检组织。7名健康受试者的口腔黏膜作为对照。在标准免疫组织化学程序中使用单克隆抗体（mAbs）来可视化表达CD1a、Langerin和CD83分子的细胞。
与健康口腔上皮相比，OLP上皮中CD1a +和Langerin +细胞的频率显著更高（分别为p <0.01和p <0.05）；然而，CD83 +细胞的频率没有差异（p> 0.05）。与健康结缔组织相比，OLP病变中的结缔组织显示CD1a +、Langerin +和CD83 +细胞的频率显著更高（p <0.01、p <0.01和p <0.05）。OLP和健康上皮中的CD1a +和Langerin +细胞具有树突状形态。
该研究表明，与健康对照相比，OLP中表达CD1a和Langerin的LCs数量增加。在结缔组织中，具有树突状形态的CD83 +细胞定位于淋巴细胞聚集区域。OLP病变结缔组织中淋巴细胞聚集区域存在CD83 +树突状细胞表明存在持续的自身抗原呈递的可能性。
