海桐隐病毒1：利用新一代测序技术完成基因组序列测定
Pittosporum cryptic virus 1: genome sequence completion using next-generation sequencing.
作者信息
Elbeaino Toufic, Kubaa Raied Abou, Tuzlali Hasan Tuna, Digiaro Michele
机构信息
Istituto Agronomico Mediterraneo di Bari, Via Ceglie 9, 70010, Valenzano (Bari), Italy.
Department of Plant Protection, Faculty of Agriculture, University of Çanakkale Onsekiz Mart, 17020, Canakkale, Turkey.
出版信息
Arch Virol. 2016 Jul;161(7):2039-42. doi: 10.1007/s00705-016-2860-5. Epub 2016 Apr 18.
Next-generation sequencing (NGS) was applied to dsRNAs extracted from an Italian pittosporum plant infected with pittosporum cryptic virus 1 (PiCV1). NGS allowed assembly of the full genome sequence of PiCV1, comprising dsRNA1 (1.9 kbp) and dsRNA2 (1.5 kbp), which encode the RNA-dependent RNA polymerase and capsid protein genes, respectively. Phylogenetic and sequence analyses confirmed that PiCV1 is a new member of the genus Deltapartitivirus, family Partiviridae. From the same plant, NSG also permitted assembly of the complete genome sequence of eggplant mottled dwarf virus (EMDV), which shared 86 % to 98 % nucleotide sequence identity with complete and partial sequences (ca 6750 nt) of other known EMDV isolates with sequences available in the GenBank database.
下一代测序（NGS）技术被应用于从一株感染了海桐隐性病毒1（PiCV1）的意大利海桐植株中提取的双链RNA（dsRNA）。NGS技术使得PiCV1的全基因组序列得以组装，该基因组由dsRNA1（1.9千碱基对）和dsRNA2（1.5千碱基对）组成，它们分别编码依赖RNA的RNA聚合酶和衣壳蛋白基因。系统发育和序列分析证实，PiCV1是双分体病毒属（Partiviridae科）的一个新成员。从同一植株中，NGS技术还使得茄子斑驳矮缩病毒（EMDV）的完整基因组序列得以组装，该序列与GenBank数据库中其他已知EMDV分离株的完整和部分序列（约6750个核苷酸）的核苷酸序列同一性为86%至98%。
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