Blawid R, Stephan D, Maiss E
Faculty of Natural Sciences, Institute of Plant Diseases and Plant Protection, Leibniz Universität Hannover, Hannover, Germany.
Arch Virol. 2007;152(8):1477-88. doi: 10.1007/s00705-007-0966-5. Epub 2007 May 29.
After extraction of double-stranded (ds) RNAs from Vicia faba, dsRNA1 and dsRNA2 of Vicia cryptic virus (VCV), a member of the genus Alphacryptovirus (family Partitiviridae), were detected in six out of seven different cultivars by agarose gel electrophoresis. In attempts to sequence the complete VCV genome, the dsRNA1 and dsRNA2 sequences from a total of five different V. faba cultivars were determined. Analysis of these sequences indicated that V. faba cultivars contain almost indistinguishable VCV sequences. The larger dsRNA1 was 2012 bp in length and contained a major open reading frame (ORF) encoding a putative RNA-dependent RNA polymerase (RdRp). The smaller dsRNA2 was 1779 bp in length and comprised a single ORF on its plus-strand encoding the coat protein (CP). The sequences of the dsRNA1 and dsRNA2 ORFs shared highest amino acid sequence identities (84 and 56%, respectively) with the corresponding gene products of the alphacryptovirus white clover cryptic virus 1 (WCCV-1). The 5'-terminal untranslated regions of dsRNA1 and dsRNA2 of VCV were highly conserved and were strikingly similar to the corresponding regions of WCCV-1. RdRp amino acid sequence alignments revealed conserved motifs, which correlate with the phylogenetic clustering of the family Partitiviridae.
从蚕豆中提取双链（ds）RNA后，通过琼脂糖凝胶电泳在7个不同品种中的6个中检测到了蚕豆隐病毒（VCV）的dsRNA1和dsRNA2，VCV是α隐病毒属（多分体病毒科）的一个成员。在尝试对完整的VCV基因组进行测序时，测定了来自总共5个不同蚕豆品种的dsRNA1和dsRNA2序列。对这些序列的分析表明，蚕豆品种含有几乎无法区分的VCV序列。较大的dsRNA1长度为2012 bp，包含一个主要的开放阅读框（ORF），编码一个假定的RNA依赖RNA聚合酶（RdRp）。较小的dsRNA2长度为1779 bp，在其正链上包含一个单一的ORF，编码外壳蛋白（CP）。dsRNA1和dsRNA2 ORF的序列与α隐病毒白三叶草隐病毒1（WCCV-1）的相应基因产物具有最高的氨基酸序列同一性（分别为84%和56%）。VCV的dsRNA1和dsRNA2的5'端非翻译区高度保守，并且与WCCV-1的相应区域惊人地相似。RdRp氨基酸序列比对揭示了保守基序，这与多分体病毒科的系统发育聚类相关。
