Yang G, Zheng H X, Wu L N, Wan H L, Li N, Yang G C, Liang J F
Department of Gerenal Surgery, Shanxi Medical University and Shanxi Tumor Hospital, Taiyuan 030001, China.
Department of Pathology, First Hosptial of Shanxi Medical University, Taiyuan 030001, China.
Zhonghua Zhong Liu Za Zhi. 2016 Apr;38(4):283-8. doi: 10.3760/cma.j.issn.0253-3766.2016.04.008.
To investigate the expression of hepatocyte growth factor (HGF) and its relationship with microsatellite instability (MSI) and their influence on survival in patients with colorectal cancer.
Immunohistochemistry (IHC) was used to detect the expression of HGF and MSI in 98 specimens of colorectal cancer. Tumors lacking protein expression of any of the four mismatch repair genes (MLH1, PMS2, MSH2 or MSH6) were labelled as MSI, and the rest were considered as microsatellite stable (MSS). The associations between expression and clinicopathological factors were assessed using Chi-square tests. Kaplan-Meier curves, log-rank test, and Cox regression were used to analyze the association between biomarker expressions and overall survival.
The incidence rate of MSI in 98 colorectal specimens was 32.7%, and was statistically significantly correlated with the location of tumor and differentiation degree (P<0.05). The HGF-expression rate was 71.4%. The patients with an MSI tumor had a significantly higher HGF expression, compared with the patients with an MSS tumor (P=0.048). The 5-year survival rate of MSI group and MSS group were 39.8% and 58.7%, respectively (P=0.009). The 5-year survival rate of HGF-positive group and HGF-negative group were 46.2% and 67.9% (P=0.035). The multivariate analysis showed that lymphocytic infiltration, TMN stage, MSI and HGF are independent prognostic factors in colorectal cancer (P<0.05 for all).
HGF is highly expressed in colorectal cancer patients with microsatellite instability. Both microsatellite instability and HGF are independent factors affecting the prognosis in patient with colorectal cancer.
探讨肝细胞生长因子（HGF）的表达及其与微卫星不稳定性（MSI）的关系，以及它们对结直肠癌患者生存的影响。
采用免疫组织化学（IHC）法检测98例结直肠癌标本中HGF和MSI的表达。缺乏四种错配修复基因（MLH1、PMS2、MSH2或MSH6）中任何一种蛋白表达的肿瘤被标记为MSI，其余的被认为是微卫星稳定（MSS）。使用卡方检验评估表达与临床病理因素之间的关联。采用Kaplan-Meier曲线、对数秩检验和Cox回归分析生物标志物表达与总生存之间的关联。
98例结直肠癌标本中MSI的发生率为32.7%，与肿瘤位置和分化程度具有统计学显著相关性（P<0.05）。HGF表达率为71.4%。与MSS肿瘤患者相比，MSI肿瘤患者的HGF表达显著更高（P=0.048）。MSI组和MSS组的5年生存率分别为39.8%和58.7%（P=0.009）。HGF阳性组和HGF阴性组的5年生存率分别为46.2%和67.9%（P=0.035）。多因素分析显示，淋巴细胞浸润、TMN分期、MSI和HGF是结直肠癌的独立预后因素（均P<0.05）。
HGF在微卫星不稳定的结直肠癌患者中高表达。微卫星不稳定性和HGF都是影响结直肠癌患者预后的独立因素。
