Garcia Tania, Lafuente Daisy, Blanco Jordi, Sánchez Domènec J, Sirvent Juan J, Domingo José L, Gómez Mercedes
Laboratory of Toxicology and Environmental Health, School of Medicine, IISPV, "Rovira i Virgili" University, Reus, Catalonia, Spain; Biochemistry Unit, School of Medicine, IISPV, "Rovira i Virgili" University, Reus, Catalonia, Spain.
Laboratory of Toxicology and Environmental Health, School of Medicine, IISPV, "Rovira i Virgili" University, Reus, Catalonia, Spain; Physiology Unit, School of Medicine, IISPV, "Rovira i Virgili" University, Reus, Catalonia, Spain.
Food Chem Toxicol. 2016 Jun;92:177-87. doi: 10.1016/j.fct.2016.04.010. Epub 2016 Apr 21.
Because of their extremely small size, silver nanoparticles (AgNPs) show unique physical and chemical properties, with specific biological effects, which make them particularly attractive for being used in a number of consumer applications. However, these properties also influence the potential toxicity of AgNPs. In this study, we assessed the potential toxic effects of an in vivo oral sub-chronic exposure to polyvinyl pyrrolidone coated AgNPs (PVP-AgNPs) in adult male rats. We also assessed if oral PVP-AgNPs exposure could alter the levels of various metals (Fe, Mg, Zn and Cu) in tissues. Rats were orally given 0, 50, 100 and 200 mg/kg/day of PVP-AgNPs. Silver (Ag) accumulation in tissues, Ag excretion, biochemical and hematological parameters, metal levels, as well as histopathological changes and subcellular distribution following PVP-AgNPs exposure, were also investigated. After 90 days of treatment, AgNPs were found within hepatic and ileum cells. The major tissue concentration of Ag was found in ileum of treated animals. However, all tissues of PVP-AgNPs-exposed animals showed increased levels of Ag in comparison with those of rats in the control group. No harmful effects in liver and kidney, as well as in biochemical markers were noted at any treatment dose. In addition, no hematological or histopathological changes were found in treated animals. However, significant differences in Cu and Zn levels were found in thymus and brain of PVP-AgNPs-treated rats.
由于其尺寸极小，银纳米颗粒（AgNPs）呈现出独特的物理和化学性质，并具有特定的生物学效应，这使得它们在许多消费应用中极具吸引力。然而，这些性质也会影响AgNPs的潜在毒性。在本研究中，我们评估了成年雄性大鼠经口亚慢性暴露于聚乙烯吡咯烷酮包覆的AgNPs（PVP-AgNPs）后的潜在毒性作用。我们还评估了经口暴露于PVP-AgNPs是否会改变组织中各种金属（铁、镁、锌和铜）的水平。给大鼠经口给予0、50、100和200mg/kg/天的PVP-AgNPs。我们还研究了PVP-AgNPs暴露后组织中的银（Ag）积累、Ag排泄、生化和血液学参数、金属水平，以及组织病理学变化和亚细胞分布。治疗90天后，在肝脏和回肠细胞中发现了AgNPs。在接受治疗的动物的回肠中发现了主要组织浓度的Ag。然而，与对照组大鼠相比，暴露于PVP-AgNPs的动物的所有组织中的Ag水平均有所升高。在任何治疗剂量下，均未发现对肝脏和肾脏以及生化标志物有有害影响。此外，在接受治疗的动物中未发现血液学或组织病理学变化。然而，在PVP-AgNPs处理的大鼠的胸腺和脑中发现了铜和锌水平的显著差异。
